Physiological response of transgenic atpA and atpB genes Arabidopsis to abiotic stress
The aim of this experiment was to investigate the growth,development,photosynthesis and energy metabolism response of transgenic atpA and atpB Arabidopsis genes under stress(drought,salt stress,cold stress).The activities of superoxide dismutase(SOD),adenosine triphosphatase(ATPase),peroxidase(POD),catalase(CAT),and the content of adenine riboside triphosphate(ATP)were determined by enzymolysis method.The physiological changes of transgenic atpA and atpB genes Arabidopsis were systematically evaluated under different PEG-6000 concentrations(0,15%,20%,25%,30%),salt concentrations(0,100,200,250,300 mmol/L)and cold stress(4℃).The results showed that with the increase of cold stress time,the ATP content of transgenic atpA Arabidopsis first increased and then decreased,and the ATP content of transgenic atpB Arabidopsis first decreased and then remained stable.Under 200 and 250 mmol/L NaCl stress,the ATP content in transgenic atpA Arabidopsis was the highest after 3 hours of stress.Under 250 and 300 mmol/L NaCl stress,the activity of ATPase in transgenic atpB Arabidopsis showed a trend of increasing first and then gradually decreasing with the extension of stress time.Under 15%,20%,and 25%PEG-6000 stress,the ATPase activity in transgenic atpA Arabidopsis gradually decreased from 3 to 12 hours and then gradually increased after 12 hours.The results show that there are significant differences in the physiological response of transgenic atpA and atpB gene Arabidopsis under stress,which may be related to the specific function and stress adaptation mechanism after gene modification.It can provide reference for the improvement of stress resistance of transgenic crops.
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