A-FABP基因在三个品种鸡中的群体遗传多态性分析
Analysis of population genetic polymorphism of the A-FABP gene in three varieties of chicken
陈芝媛 1苏咏梅 1朱文进 1宋雪柔 1张铷铄 1代晓琳1
作者信息
- 1. 河北科技师范学院动物科技学院,河北 秦皇岛 066004
- 折叠
摘要
试验旨在探究脂肪细胞型脂肪酸结合蛋白(A-FABP)基因在坝上长尾鸡、东乡黑鸡和白羽来航鸡3个品种中的遗传多态性.试验采用单链构象多态性分析(PCR-SSCP)方法对3个品种鸡的A-FABP基因进行SNP检测.结果显示,共发现4个突变位点:g.09A>G、g.110T>C、g.1607A>G和g.1727A>C,均为在鸡中首次发现的突变位点,且g.1607A>G和g.1727A>C两个位点的突变只发生在坝上长尾鸡.坝上长尾鸡多态信息含量(PIC平均值为0.328 0)和观测杂合度(Ho平均值为0.594 5)在3个品种中均为最高.Hardy-Weinberg平衡性检验发现坝上长尾鸡有3个突变位点(g.09A>G、g.1607A>G和g.1727A>C)处于平衡状态(P>0.05),白羽来航鸡的2个突变位点(g.09A>G和g.110T>C)处于平衡状态(P>0.05),其余各位点处于不平衡状态(P<0.05).研究表明,A-FABP基因在坝上长尾鸡的遗传多态性高于东乡黑鸡和白羽来航鸡.
Abstract
The experiment aimed to investigate the population genetic polymorphism of fat adipocyte type fatty acid binding protein(A-FABP)gene among Bashang long-tail chicken,Dongxiang black chicken,and Baiyu Leghorn chicken.The SNP detection of A-FABP gene sequences in different breeds were carried out by the PCR-SSCP method.The results showed that there were four mutation sites:g.09A>G,g.110T>C,g.1607A>G,and g.1727A>C,all of which were first discovered in chickens.The mutations at the two loci of g.1607A>G and g.1727A>C occurred only in the Bashang long-tail chicken,and Bashang long-tail chickens had the highest polymorphic information content(mean PIC value 0.328 0),and the highest observed heterozygosity(average Ho 0.594 5).Hardy-Weinberg equilibrium test found that three mutation sites(g.09A>G,g.1607A>G and g.1727A>C)in Bashang long-tail chickens were in equilibrium(P>0.05),and two mutation sites(g.09A>G,g.110T>C)in Baiyu Leghorn chicken were in equilibrium.(P>0.05),and the other sites are in an unbalanced state(P<0.05).The study indicates that the genetic polymorphism of A-FABP gene in Bashang long-tailed chicken was higher than that in Dongxiang black chicken and Baiyu Leghorn chicken.
关键词
地方品种鸡/A-FABP/PCR-SSCP/群体遗传多态性Key words
local breed chicken/A-FABP/PCR-SSCP/population genetic polymorphism引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据