混菌固态发酵降解蒸汽爆破芦苇秸秆纤维素的研究
Study on degradation of cellulose from steam-blasted reed straw by solid-state fermentation with mixed bacteria
朱龙博 1韩倩男 1申娜娜 2刘正群 2原雪峰 3李宁 2闫峻 2孙超4
作者信息
- 1. 西北农林科技大学动物科技学院,陕西 杨凌 712100;天津市农业科学院畜牧兽医研究所 天津市畜禽分子育种与生物技术重点实验室 天津市畜禽健康养殖工程技术中心,天津 300381
- 2. 天津市农业科学院畜牧兽医研究所 天津市畜禽分子育种与生物技术重点实验室 天津市畜禽健康养殖工程技术中心,天津 300381
- 3. 天津现代天骄农业科技股份有限公司 天津市绿色生态饲料重点实验室,天津 301800
- 4. 西北农林科技大学动物科技学院,陕西 杨凌 712100
- 折叠
摘要
为提高芦苇秸秆利用率,试验采用3株产朊假丝酵母菌(CR1、CR2和CR3).1株枯草芽孢杆菌(KC)、1株酿酒酵母菌(NJ)和1株植物乳杆菌(ZR)对芦苇秸秆进行单菌固态发酵,并对CR1、CR2、CR3进行耐高氮菌株的诱导筛选.在此基础上,采用单因素试验对菌种组合、菌种比例、复合菌接种量、氮源种类等进行筛选优化,探究其对芦苇秸秆混菌固态发酵的影响.结果显示,CR3菌株的耐高氮能力较强,液体(2.5%尿素)培养后,CR3菌浓度≥107 CFU/mL.芦苇秸秆最佳发酵工艺为:菌种组合及比例为CR3∶NJ∶ZR=2∶1∶1,复合菌接种量为15%,尿素为最佳氮源.此条件下,粗蛋白和真蛋白含量分别为14.15%和9.74%,较发酵前分别增加76.65%和67.35%;纤维素及半纤维素降解率分别为21.07%和27.30%,较发酵前分别增加68.56%和167.65%.研究表明,混菌固态发酵可增加芦苇秸秆的粗蛋白含量,降低粗纤维含量.
Abstract
In order to improve the utilization rate of reed straw,three strains of prion-producing Candida(CR1,CR2 and CR3),one strain of Bacillus subtilis(KC),one strain of Saccharomyces cerevisiae(NJ)and one strain of Lactobacillus plantarum(ZR)were used for single-bacterial solid-state fermentation,and CR1,CR2 and CR3 strains with high nitrogen tolerance were induced and screened.On this basis,the single-factor test method was used to screen and optimize the strain combination,strain proportion,composite bacteria inoculated amount,nitrogen source type,etc.,to explore their influence on the solid-state fermentation of reed straw with mixed bacteria.The results showed that the CR3 strain was more tolerant to high nitrogen,and the CR3 bacterial concentration was≥107 CFU/mL after liquid(2.5%urea)cultivation.The optimal strain combination and ratio was CR3∶NJ∶ZR=2∶1∶1,the inoculum amount of the composite bacteria was 15%,and urea was the optimal nitrogen source,and the content of crude protein and true protein under these conditions was 14.15%and 9.74%,respectively,which were 76.65%and 67.35%higher than before fermentation.The degradation rates of cellulose and hemicellulose were 21.07%and 27.30%,respectively,which were 68.56%and 167.65%higher than before fermentation.The study indicates that solid-state fermentation with mixed bacteria can increase the crude protein content and reduce the crude fiber content of reed straw.
关键词
混菌固态发酵/蒸汽爆破/芦苇秸秆/高氮诱导/纤维素Key words
solid-state fermentation with mixed bacteria/steam blasting/reed straw/high nitrogen induction/cellulose引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据