目的 探究TBX2基因与头颈鳞癌(head and neck squamous cell carcinoma,HNSCC)的关系,分析甲基化预后的价值.方法 综合利用UCSC Xena数据库、基因表达综合(Gene Expression Omnibus,GEO)数据库、人类蛋白质图谱(Human Protein Atlas,HPA)数据库、UALCAN数据库挖掘TBX2 基因的表达、DNA甲基化和临床相关性,利用基因集富集分析(gene set enrichment analysis,GSEA)法富集TBX2 在HNSCC中所参与的信号通路.结果 TBX2 基因在HNSCC中高表达,TBX2 基因高、低表达组HNSCC患者的总生存率(overall survival,OS)无明显差异,但HNSCC中TBX2 基因的表达与临床分级分期有关.TBX2 基因某些cg位点的低甲基化水平与较差的OS和无进展生存期(progression free survival,PFS)密切相关.GSEA结果显示,TBX2基因主要参与钙信号通路、黏着斑、蛋白酶体等多条通路.结论 TBX2 基因可作为HNSCC特异性诊断和预后的生物标志物.
Abstract
Objective To explore the relationship between TBX2 gene and head and neck squamous cell carcinoma(HNSCC),and to analyze the value of methylation prognosis.Methods The UCSC Xena database,Gene Expression Omnibus(GEO)database,Human Protein Atlas(HPA)database and UALCAN database were comprehensively used to explore the expression,DNA methylation and clinical correlation of TBX2 gene,and gene set enrichment analysis(GSEA)method was used to enrich the signaling pathways involved in TBX2 gene in HNSCC.Results TBX2 gene was highly expressed in patients with HNSCC,and there was no significant difference in overall survival(OS)in patients with HNSCC in TBX2 gene high expression group and low expression group.However,the expression of TBX2 in HNSCC was related to clinical grade and stage.Low methylation levels at certain cg sites of the TBX2 gene were closely associated with poor OS and progression free survival(PFS).The results of GSEA showed that TBX2 gene was mainly involved in calcium signaling pathway,adhesion plaque,proteasome and other pathways.Conclusion TBX2 gene can be a biomarker for the specific diagnosis and prognosis of HNSCC.
关键词
TBX2基因/甲基化/头颈鳞癌/预后
Key words
TBX2/Methylation/Head and neck squamous cell carcinoma/Prognosis
维普
万方数据