铜死亡相关长链非编码RNA预测喉癌的预后和调控机制
Prognosis prediction and regulatory mechanism of cuproptosis-associated long non-coding RNA in laryngeal cancer
李淑桢 1昝雨欣1
作者信息
- 1. 湖北医药学院生物医药研究院(湖北十堰 442000)
- 折叠
摘要
目的 探讨铜死亡相关的长链非编码RNA(long non-coding RNA,lncRNA)在喉癌临床预后预测和免疫治疗中的作用.方法 使用癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库中的基因组和临床数据,通过LASSO分析和Cox回归分析构建预后模型,使用主成分分析、时间依赖性受试者工作特征(receiver operating characteristic,ROC)曲线和一致性指数评估其临床效能;从肿瘤突变负荷、肿瘤免疫功能障碍和排除、肿瘤干性指数等多个维度分析风险模型的预后价值;最后通过基因集富集分析(gene set enrichment analysis,GESA)探索模型高风险组的细胞功能和通路富集.结果 共得到5个与铜死亡相关的lncRNA预后模型.主成分分析、ROC曲线下面积(area under the curve,AUC)超过0.8、一致性指数表明所建立的风险模型具备出色的预测能力;在高低风险患者中,风险曲线与免疫相关功能无显著相关性,与RNA干性评分呈负相关(r=-0.21,P=0.025);富集分析表明,lncRNA的生物学功能与WNT信号通路有关.结论 铜死亡相关的lncRNA可能是一种新的预测喉癌预后的生物标志物,并可能为喉癌的治疗提供新途径.
Abstract
Objective To investigate the role of long non-coding RNA(lncRNA)associated with cuproptosis in clinical prognosis prediction and immunotherapy of laryngeal cancer.Methods Genomes and clinical data from The Cancer Genome Atlas(TCGA)database were used to construct prognostic models by LASSO analysis and Cox regression analysis.Principal component analysis,time-dependent receiver operating characteristic(ROC)curve,and consistency index were used to evaluate their clinical efficacy.The prognostic value of the risk model was analyzed in terms of tumor mutation load,tumor immune dysfunction and exclusion,and stemness index.Finally,gene set enrichment analysis(GESA)was used to explore the cell function and pathway enrichment in high-risk model group.Results Five prognostic models of lncRNA associated with cuproptosis were obtained.Principal component analysis,area under the curve(AUC)exceeding 0.8 for ROC curve,and the concordance index demonstrated the superior predictive capability of the established risk models.In high-and low-risk patients,the risk curves were not significantly correlated with immune-related function,but were negatively correlated with RNA stemness scores(r=-0.21,P=0.025).Enrichment analysis showed that the biological function of lncRNA was related to WNT signaling pathway.Conclusion lncRNA associated with cuproptosis may be a new biomarker to predict the prognosis of laryngeal cancer,and may provide a new approach for the treatment of laryngeal cancer.
关键词
铜死亡/长链非编码RNA/喉癌/预后/免疫治疗Key words
Cuproptosis/lncRNA/Laryngeal cancer/Prognosis/Immunotherapy引用本文复制引用
出版年
2024
NETL
NSTL
维普
万方数据