Determination of Contents of Antioxidant 405 in Compound Additives by High-performance Liquid Chromatography(HPLC)
A method was established to determine the antioxidant 405 contents in compound additives by using high-performance liquid chromatography(HPLC).AgilentODS-C18(4.6 mm×150 mm,5 μm)was adopted with the mobile phase of methanol at a flow rate of 1.2 mL/min.The column temperature was at 40℃.The detection wavelengths was at 250 nm.The injection volume was 5 μL.Results showed that the method had a good linear relationship within the range of 0.3～2.4 mg/mL for antioxidant 405,with the correlation coefficient r of 1.000 0.The recovery rates of antioxidant 405 samples were 100%～101%with different concentrations.This method is accurate,convenient and rapid so as to be used for determining the antioxidant 405 contents in compound additives.
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