Advances in CRISPR/Cas9 mediated gene knock-in techniques of zebrafish
As an adaptive immune defense system in bacteria and archaea,clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9(CRISPR/Cas9)has been applied to precise gene editing in many species.Given the zebrafish has a lot of advantages including short spawning cycle,easy feeding,high fecundity and convenient observation of transparent embryo,many studies based on gene editing have been carried out using zebrafish as an significant vertebrate developmental biological model organism.Compared with the high efficiency of random mutation by gene knock-out(KO),the low efficiency of precise gene knock-in(KI)has always been a shortcoming in the field of zebrafish gene editing.This paper reviewed the research of CRISPR/Cas9 system for targeted KI in zebrafish,and provides reference for the optimization of precise KI efficiency and the establishment of zebrafish disease models.
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维普
