In view of the disadvantages of the traditional teaching mode,we explore the reform and innovation of the"biochemistry"teaching mode under the guidance of new engineering construction and OBE(oulcome-based education)concept.It is analyzed from four aspects:improving students′ participation,improving teaching quality,optimizing teaching content and strengthening students′ practical ability.The teaching mode of"one center,two classrooms,three integrations,and application-oriented"is constructed in order to improve the teaching quality and promote the cultivation of application-oriented engineering talents with international competitiveness.