Virus infection is initiated by the interaction between the viral attachment protein and the surface structure of the host cell.Cell receptors are the key for viruses to invade the hosts,so developing high throughput and accurate viral receptor identification methods is crucial for the prevention and control of viral diseases.In recent years,various technologies have promoted the identification of viral receptors which can be broadly divided into two categories:genomic-based and interactome-based approaches.Genomic-based approaches include CRISPR/Cas9 knockout,CRISPR activation,cDNA libraries,RNA interference,and random insertion mutagenesis.Interactome-based approaches include protein microarray,affinity purification-mass spectrometry,and cross-linking mass spectrometry.This review attempts to focus on analyzing relevant technologies based on genome and interactome,summarizing their applications,advantages and disadvantages,in order to provide a reference for the further development of virus receptor identification technology.
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