We have established three assessment modules consisting of ″regular assessment″ ″advanced assessment″and″advanced highly assessment″,and applied them to offline teaching,forming the Biochemistry course assessment and evaluation system,aiming at solving the problems existing in the assessment of Biochemistry courses guided by the concept of OBE and combined with the principle of formative assessment.This diversified assessment and evaluation system focuses on process evaluation and students′usual learning effects,aiming to stimulate students′ enthusiasm for learning and give full play to students′ subjective initiative.The implementation of the assessment model has had a positive impact on both students and teachers,not only providing students with rules to follow,which is conducive to the comprehensive achievement of their knowledge,ability,and quality goals,but also providing teachers with evidence to rely on,striving to achieve openness,fairness,impartiality,and rationality,which is conducive to the continuous improvement and vigorous promotion of the teaching quality of Biochemistry courses.Meanwhile,it also provides a reference for the reform of the assessment mode of Biochemistry and other courses in other domestic colleges and universities.