The research progress on genetic transformation system of filamentous fungi
With the rapid development of modern sequencing technology,the genome sequences of major filamentous fungi have been published one after another,and the research on the gene level of filamentous fungi has also been deepened.The development of efficient genetic transformation technology is the premise of fungal gene function research and targeted improvement of strains.In recent years,researchers have continuously optimized and developed new genetic transformation methods.However,there are still some problems,such as low transformation efficiency and difficulty in screening positive transformants.Suitable transformation methods and screening markers play an important role in the genetic transformation of filamentous fungi.The research progress of filamentous fungi genetic transformation system is reviewed in this paper,mainly from the aspects of the treatment of recipient cells,integration and expression of foreign genes,common transformation methods and screening and identification of positive transformants in order to provide ideas for the construction of filamentous fungi genetic transformation system.
万方数据
维普
