The protecting effects of Pediococcus acidilactici on the gut microbiota dysbiosis induced by antibiotic-associated diarrhea
Although the widespread use of antibiotics can effectively prevent and treat infections caused by bacteria,antibiotics can also reduce the richness and diversity of intestinal microbiota.In order to investigate the protecting effects of probiotics on intestinal microbiota dysbiosis caused by antibiotic associated diarrhea(AAD),the AAD mice model was established by oral administration of amoxicillin and then were treated with intragastric administration of Pediococcus acidilactici.High throughput sequencing technology was used to analyze the communities of intestinal microbiota.The alpha diversity analyses indicated that oral administration of antibiotics could reduce the richness and diversity of the intestinal flora in mice,however the richness and diversity of the intestinal flora of mice could be enhanced by the intragastric administration of P.acidilactici.The Beta diversity analyses indicated that the gut microbiota of the three groups of mice had their own characteristics and were separated into different taxonomic clusters.At the genus level,the proportions of beneficial bacteria such as Lactobacillus,Faecalibacterium and Lachnoclostridium in the antibiotic treated mice were lower than those of the control group.However,the proportions of these beneficial bacteria in the P.acidilactici treatment group were significantly increased.In addition,the proportion of opportunistic pathogens such as Staphylococcus,Klebsiella,Corynebacterium and Escherichia-Shigella in the in the P.acidilactici treatment group were lower than those in the antibiotic treatment group.Therefore,the current results indicated that antibiotic treatment could disturb the balance of gut microbiota,while P.acidilactici could repair and restore the balance of gut microbiota.The differences in microbial communities were further analyzed,and the relative abundances of Klebsiella,Faecalibaculum,Enterorhabdus,Turicibacter,Yaniella,Aerococcus,Alistipes,and Brachybacterium were significantly different.In all,the current study demonstrated that P.acidilactici showed prospective protecting effects on intestinal microbiota dysbiosis caused by AAD,and the repairing mechanism might be related with the certain critical species in the intestinal microbiota.
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