Changes of mitochondrial fusion/fission proteins and their relationship with apoptosis during adipose-derived stromal cells differentiation into astrocytes
Adipose-derived stromal cells(ADSC)were induced into astrocytes(AS)in vitro and divided into 48 h,7 d,14 d,and 21 d groups according to the induction time.Immunocytochemical method was used to detect the expression of glial fibrillary acidic protein(GFAP),mitochondrial fusion protein 2(Mfn2),mitochondrial fission 1(Fis1),cysteine aspartate specific protease-3(Caspase-3).The survival status of cells in each group was detected by flow cytometry using Annexin Ⅴ/PI double staining method to investigate the changes of mitochondrial morphological regulatory proteins(mitochondrial fusion,mitochondrial fission proteins)during ADSC induced differentiation into AS and their relationship with apoptosis.The results showed that during ADSC induced differentiation into AS,the positive expression rate of Mfn2 gradually decreased with the prolongation of induction time,and reached the lowest level at the 14th day of induction;the positive expression rates of Fis1 and Caspase-3 gradually increased with the prolongation of induction time,reaching a peak at 14 days of induction;The survival rate of cells gradually decreases,while the apoptosis rate gradually increases.The results suggest that during the induction of ADSC into AS,the insufficient expression of Mfn2 and the overexpression of Fis1 may lead to an increase in the expression of apoptosis related protein Caspase-3,leading to the occurrence of cell apoptosis,as the induction time prolongs.
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维普
