The research of siRNA drugs is in the ascendant.To investigate the effects of different chemical modifications and storage methods on the validity period of siRNA,unmodified(siRNA-G),thio-modified(siRNA-T1)and combinatorially modified(thio+locked nucleotide+methoxy group:siRNA-M8)siRNAs were stored under three different conditions,namely raw siRNA solution at-20℃,freeze-dried siRNA powder at-20℃or room temperature.The different testing results showed that validity period was 60 days for raw siRNA solution at-20℃,120 and 60 days for freeze-dried siRNA powder at-20℃and room temperature,respectively,while the ways of chemical modifications had neglectful influence on the validity period.The initial purity of siRNA-G,siRNA-M8 and siRNA-T1 are 98.51%,98.90%and 97.20%by HPLC and GC,respectively.During the best valid life,all kinds of siRNA were detected as sterile by potato dextrose agar(PDA)method.The knock down level of intracellular target gene was greater than 35%within valid period.The Tm value is stable during the validity period.The sequence characterization is qualified.The current data provided an important reference for the rational and effective use of siRNA.
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