Multidimensional analysis of the shelf life of siRNA storage
The research of siRNA drugs is in the ascendant.To investigate the effects of different chemical modifications and storage methods on the validity period of siRNA,unmodified(siRNA-G),thio-modified(siRNA-T1)and combinatorially modified(thio+locked nucleotide+methoxy group:siRNA-M8)siRNAs were stored under three different conditions,namely raw siRNA solution at-20℃,freeze-dried siRNA powder at-20℃or room temperature.The different testing results showed that validity period was 60 days for raw siRNA solution at-20℃,120 and 60 days for freeze-dried siRNA powder at-20℃and room temperature,respectively,while the ways of chemical modifications had neglectful influence on the validity period.The initial purity of siRNA-G,siRNA-M8 and siRNA-T1 are 98.51%,98.90%and 97.20%by HPLC and GC,respectively.During the best valid life,all kinds of siRNA were detected as sterile by potato dextrose agar(PDA)method.The knock down level of intracellular target gene was greater than 35%within valid period.The Tm value is stable during the validity period.The sequence characterization is qualified.The current data provided an important reference for the rational and effective use of siRNA.
万方数据
维普
