Rapid detection of Campylobacter jejuni based on PCR-immune strip method
Food-borne diarrheal disease caused by Campylobacter jejuni(C.jejuni)has become one of the prominent public health problems.Rapid detection of C.jejuni contamination in food is of great significance for protecting human health.In this study,the lpxA gene sequence of C.jejuni was used as the target gene,a PCR-test strip detection method was established by binding digoxin and isothiocyanate(ITC)molecules labeled on polymerase chain reaction(PCR)products to antibodies on the strip.All standard strains of C.jejuni were positive by our method,while other Campylobacter species and food-borne pathogens were negative result.The detection specificity was 100%,and the detection limit was 3.1×103 CFU/mL.This method is consistent with the results of the current national standard detection methods.The application of this method for the detection of C.jejuni would reduce the cost,simplify the operation procedure,and provide a practical and easy method for the detection of food-borne C.jejuni.
Campylobacter jejunipolymerase chain reactionimmune test strip
万方数据
维普
