DNA-methylation modification in somatic cell nuclear transfer
Reprogramming of a somatic nucleus to a totipotential state of supporting embryonic development is thought to be essential for successful cloning by SCNT (somatic cell nuclear transfer). Due to the evolved capacity of oocyte in normal fertilization, SCNT-induced reprogramming of a differentiated nucleus to a totipo-tential state is suboptimal in comparison with adequate and appropriately timed maternal and paternal genome reprogramming ensuing normal fertilization. Meanwhile, DNA-methylation pattern is one of the key factors which affect the development of SCNT-reconstructed embryos and gene expression profile. Hence, thorough understanding of the molecular mechanism underlying DNA-methylation modification and grasp of the knowl-edge about the role of DNA-demethylation in reprogramming of a donor nucleus, further enhancing the efficacy of this process and the developing potential of SCNT-reconstructed embryos are likely to have a significant impact on elevating the SCNT efficiency.
DNA methylationSCNTreconstructed embryoepigenetic reprogramming
NETL
NSTL
万方数据
维普
