Glycosylation is a conserved post-translation modification in all eukaryotes and endues protein with multiple functions. Our knowledge of glycosylation is mainly come from the investigation of yeast and mammalian cells, however, these investigations at cellular level can not tell us the complicated function of glycosylation during development of multi-cellular eukaryotes. Filamentous fungi are multi-cellular eukaryotes with a relative simple life cycle and therefore are thought to be one of ideal models for glycobiological investigation of multi-cellular eukaryotes. During last decade, glycobiology in filamentous fungi has drawn more attentions. It has been revealed that glycosylation in filamentous fungi is involved in their growth and development.