Study of the Effects of Isorhamnetin on Proliferation and Glycolysis of Oral Squamous Cell Carcinoma Cells
To investigate the effects of isorhamnetin on human oral squamous cell carcinoma(OSCC)cells,the impact of different doses of isorhamnetin on the proliferation of OSCC cell lines CAL27 and SCC9 was detected using both cell viability and colony formation assays,and the effect of isorhamnetin on lactate pro-duction and glucose consumption in OSCC cells was determined by glycolysis assay.The changes in glycoly-sis-related signaling pathways after treatment with different doses of isorhamnetin were examined through immunoblotting method.Finally,the xenograft mouse model was used to validate the in vivo inhibitory effect of isorhamnetin on tumors.The in vitro experiments showed that isorhamnetin dose-dependently inhibited the proliferation and glycolysis of CAL27 and SCC9 cells,and this inhibitory effect was related to the sup-pression of key glycolysis rate-limiting enzyme hexokinase Ⅱ(HK2)rather than phosphofructokinase(PFK)and pyruvate kinase M2(PKM2).The in vivo experiments demonstrated that isorhamnetin inhibited the growth of OSCC and reduced the expression of Ki67 and HK2 in tumor tissue.Overall,the results suggest that isorhamnetin inhibits the proliferation and glycolysis of OSCC,and the inhibitory effect is associated with the downregulation of HK2 expression.
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