Effect of High Glucose Acclimation on the Ability of Umbilical Cord Mesenchymal Stem Cells to Resist High Glucose Environment
In order to explore the high glucose environment resistance of human umbilical cord mesenchymal stem cells(hUC-MSCs)following high glucose acclimation,and to provide highly adaptive cells and methods for diabetes treatment,the culture media with glucose concentration gradients were used to acclimate hUC-MSCs to passage 5,and the high glucose acclimated cells(HGACs)were harvested.At the same time,the cul-ture media with 1.0 g/L glucose was used to culture hUC-MSCs to passage 5,and the low glucose cells(LGCs)were harvested.A total of 1.0×106 LGCs were cultured for 48 h in the culture media with 4.5 g/L glucose,and the high glucose cells(HGCs)were harvested.The size and proliferation of LGCs,HGCs and HGACs were measured,and the cell phenotype was detected by flow cytometry.Intracellular superoxide dismutase(SOD)activity and malondialdehyde(MDA)content were determined using xanthine oxidase and thiobarbituric acid colorimetric assays,and the expression levels of anti-aging and antioxidant genes were detected by qRT-PCR and Western-blot.The cell size of HGACs was significantly smaller than that of HGCs(P＜0.05),and the proliferation of HGACs was faster.The expression rates of CD73,CD90 and CD105 in HGACs were above 95％,and those of CD34 and CD45 were less than 2％.The SOD activity in HGACs was significantly higher than that in HGCs(P＜0.05),and the content of MDA in HGACs was lower(P＜0.05).Meanwhile,the expression levels of cellular anti-aging gene p21 and antioxidant genes Nrf2,HO-1 and NQO1 were signifi-cantly higher than those of HGCs(P＜0.05).The results demonstrated that high glucose acclimation can in-crease the activity of hUC-MSCs and make them resistant to high glucose environment.
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