免疫组织化学特异性抗体诊断浆膜腔积液转移性腺癌与增生性间皮细胞的应用价值
Application Value of Immunohistochemistry Specific Antibodies in the Diagnosis of Metastatic Adenocarcinoma And Proliferative Mesothelial Cells in Effusion in the Serous Cavity
赵维聪 1彭江兰 1牙冬雪 1陈盈 1冯敏 1韦丽洙2
作者信息
- 1. 广西华银医学检验所,广西南宁 530031
- 2. 南宁市第九人民医院检验科,广西南宁 530409
- 折叠
摘要
目的 探讨免疫组织化学特异性抗体诊断浆膜腔积液转移性腺癌(MA)与增生性间皮细胞的应用价值.方法 选取2021年1月至2023年10月广西华银医学检验所病理科浆膜腔积液标本181例,其中,113例有转移性腺癌细胞,68例有增生性间皮细胞.制备细胞石蜡切片,采用免疫组织化学标记法检测抗体绒毛蛋白(Villin)、细胞角蛋白7(CK 7)、上皮特异抗原(Ber-Ep 4)、上皮细胞表面糖蛋白单克隆抗体MOC-31)、癌胚抗原(CEA)水平,分析上述抗体诊断MA与增生性间皮细胞的应用价值.结果 Villin、CK7、Ber-Ep4、MOC-31及CEA鉴别诊断转移性腺癌细胞与增生性间皮细胞的灵敏度分别为54.87％、69.91％、78.76％、93.81％、81.42％,特异度分别为94.12％、92.65％、88.24％、92.65％、92.65％.结论 免疫组织化学特异性抗体鉴别诊断浆膜腔积液中转移性腺癌细胞与增生性间皮细胞的准确性高,在细胞病理学诊断中的应用价值较高.
Abstract
Objective:To explore the application value of immunohistochemical specific antibody in the diagnosis of metastatic adenocarcinoma(MA)and proliferative mesothelial cells in serous cavity effusion.Methods:From January 2021 to October 2023,181 cases of serous cavity effusion in pathology department of Guangxi Huayin Medical In-spection Institute were selected,among which 113 cases had metastatic gonadal cancer cells and 68 cases had prolif-erative mesothelial cells.Paraffin sections of cells were prepared,and the levels of antibody Villin,cytokeratin 7(CK 7),epithelial specific antigen(Ber-Ep 4),monoclonal antibody MOC-31 against glycoprotein on epithelial cell surface and carcinoembryonic antigen(CEA)were detected by immunohistochemical method,and the applica-tion value of these antibodies in diagnosing MA and proliferative mesothelial cells was analyzed.Results:The sensi-tivity of Villin、CK7、Ber-Ep4、MOC-31 and CEA in differential diagnosis of metastatic gonadal cancer cells and proliferative mesothelial cells were 54.87％、69.91％、78.76％、93.81％and 81.42％,and the specificity was 94.12％、92.65％and 88.24％,respectively.Conclusion:Immunohistochemical specific antibody has high accuracy in differential diagnosis of metastatic gonadal cancer cells and proliferative mesothelial cells in serous cavity effu-sion,and has high application value in cytopathological diagnosis.
关键词
浆膜腔积液/免疫组织化学/抗体/转移性腺癌细胞/增生性间皮细胞Key words
Serous cavity effusion/Immunohistochemistry/Antibody/Metastatic gonadal cancer cells/Proliferative mesothelial cell引用本文复制引用
基金项目
南宁市科技局重点研发计划项目(20193123)
出版年
2024
国家科技期刊平台
NSTL
万方数据