MiR133b-5p Regulates The EGFR/MAPK Signaling Pathway on Lung Tissue Inflammation in Rats Exposed to PM2.5
Objective:To investigate the effect of miR-133b-5p on PM2.5-induced inflammatory lung injury in rats through the EGFR/MAPK signaling pathway.Methods:48 male Wistar rats were divided into control group and three groups(low、medium and high)with 12 rats in each group.PM2.5 was poisoned by tracheal instillation.The expression levels of MUC5AC、TNF-α and IL-1 in each group were detected.The expression levels of miR-133b-5p、EGFR and MUC5AC were detected by qRT-PCR.The expression levels of EGFR、MUC5AC and Calu-din1 protein in lung tissue were detected by immunohistochemistry.The expression levels of EGFR、MUC5AC and Caludin1 in airway epithelium were detected by WesternBlotting.Results:Compared with the control group,the ser-um levels of MUC5AC、TNF-α and IL-1 in each dose group of PM2.5 were significantly increased(P<0.05);The expression levels of miR-133b-5p、EGFR and MUC5AC in lung tissue all increased(P<0.05);The expres-sion levels of MUC5AC、EGFR and MAPK in lung tissue also increased in a dose-dependent manner(P<0.05).Conclusion:PM2.5 exposure can increase the expression level of miR-133b-5p in lung tissue of rats,accompanied by the activation of EGFR and MAPK signaling pathways and the increase of MUC5AC expression level.This dis-covery provides a new idea for understanding the molecular mechanism of lung injury caused by PM2.5.
Fine particlesRatMicrorna-146aEGFR/MAPK signaling pathwayInflammatory injury
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