首页|Transcriptomic analysis of Andrias davidianus meat and experimental validation for exploring its bioactive components as functional foods

Transcriptomic analysis of Andrias davidianus meat and experimental validation for exploring its bioactive components as functional foods

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Andrias davidianus(Chinese giant salamander,CGS)is the largest and oldest extant amphibian species in the world and is a source of prospective functional food in China.However,the progress of functional peptides mining was slow due to lack of reference genome and protein sequence data.In this study,we illustrated full-length transcriptome sequencing to interpret the proteome of CGS meat and obtain 10 703 coding DNA sequences.By functional annotation and amino acid composition analysis,we have discovered various genes related to signal transduction,and 16 genes related to longevity.We have also found vast variety of functional peptides through protein coding sequence(CDS)analysis by comparing the data obtained with the functional peptide database.Val-Pro-Ile predicted by the CDS analysis was released from the CGS meat through enzymatic hydrolysis,suggesting that our approach is reliable.This study suggested that transcriptomic analysis can be used as a reference to guide polypeptide mining in CGS meat,thereby providing a powerful mining strategy for the bioresources with unknown genomic and proteomic sequences.

Chinese giant salamanderTranscriptomic analysisBioactive componentsFunctional peptides mining

Changge Guan、Zhenglin Tan、Shucheng Li、Yi Wang、Naoyuki Yamamoto、Chong Zhang、Songjun Wang、Junjie Chen、Xinhui Xing

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Key Laboratory for Industrial Biocatalysis,Ministry of Education of China,Department of Chemical Engineering,Tsinghua University,Beijing 100084,China

School of Life Science and Technology,Tokyo Institute of Technology,Yokohama 226-0026,Japan

Sensing System Research Center,National Institute of Advanced Industrial Science and Technology,Tsukuba 305-8565,Japan

Center for Synthetic and Systems Biology,Tsinghua University,Beijing 100084,China

Henan Giant Salamander Protection and Development Association,Luoyang 471000,China

Intelligent Computing Research Center,Harbin Institute of Technology,Shenzhen 518071,China

Institute of Biopharmaceutical and Health Engineering,Tsinghua Shenzhen International Graduate School,Shenzhen 518055,China

Institute of Biomedical Health Technology and Engineering,Shenzhen Bay Laboratory,Shenzhen 518067,China

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Shenzhen Science and Technology Innovation Commission

KCXFZ20201221173207022

2024

10.26599/FSHW.2022.9250014

食品科学与人类健康(英文)

食品科学与人类健康(英文)

CSTPCD
ISSN：
年,卷(期)：2024.13(1)
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