Total flavonoids of Astragalus membranaceus protect against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced neurotoxicity in mice by inhibiting ferroptosis through SLC7A11/GPX-4 signaling pathway

Zitian Gao ¹Gaorui Wang ²Yujie Chen ¹Wuke Yuan ³Jun Cai ⁴Aiping Feng ³Jie Fang ³Qi Xu ³Xiaojun Wu²

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作者信息

1. School of Public Health,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai Key Laboratory of Compound Chinese Medicines,The Ministry of Education(MOE)Key Laboratory for Standardization of Chinese Medicine,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China
2. Shanghai Key Laboratory of Compound Chinese Medicines,The Ministry of Education(MOE)Key Laboratory for Standardization of Chinese Medicine,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China
3. School of Public Health,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China
4. Longhua Hospital affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China
折叠

Abstract

Parkinson's disease(PD)is a common neurodegenerative disorder with no cure.Astragalus membranaceus is used in Chinese culture as a food supplement to boost immunity.The present study aimed to explore the neuroprotective effects of total flavonoids extracted from A.membranaceus(TFA)and their protective mechanisms.TFA offered neuroprotection against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)in the mouse model of Parkinsonism,by improving behavior performance in the gait analysis and pole test,and inhibiting the decline of tyrosine hydroxylase(TH)positive neurons and TH protein expression in substantia nigra of mice.TFA also prevented 1-methy1-4-phenylpyridinium(MPP+)induced neurotoxicity in SH-SY5Y cells,by increasing GSH and GSH/GSSG ratio,and reducing reactive oxygen species.In addition,the neuroprotective effects of TFA were associated with its ability to restore MPTP/MPP+induced downregulation of SLC7A11 and glutathione peroxidase 4(GPX-4).In conclusion,we demonstrated that TFA exerted significant neuroprotection against MPTP/MPP+induced neurodegeneration by inhibiting ferroptosis through the regulation of SLC7A11/GPX-4 axis,suggesting the use of TFA as a possible food supplement in the prevention of PD.

Key words

Parkinson's disease/Total flavonoids of Astragalus membranaceus/Ferroptosis/SLC7A11

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出版年

2024

食品科学与人类健康(英文)

CSTPCDCSCD

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