Demonstration of safety characteristics and effects on gut microbiota of Lactobacillus gasseri HMV18

扫码查看

原文链接

万方数据
维普

外文摘要：Human normal flora is a source of probiotics.The safety characteristics of a specific isolate determine its application in foods or drugs.The food-borne-pathogen antagonist strain Lactobacillus gasseri HMV18 is one of the isolates from normal human flora.In this work,we assessed the in vitro pH tolerance,bile tolerance,biogenic amine production,mucin utilization,and safety of in vivo administration to mice to evaluate general health,organ-body weight index,organ histopathological change,whether L.gasseri HMV18 can colonize in the gut or modulate the gut microbiota after oral administration.The results suggest that L.gasseri HMV18 can tolerate pH 3 for 2 h,3％bile for 3 h,biogenic amine negative,mucin usage negative,does not encode verified toxins,and cause no visible change in mice's organs.L.gasseri HMV18 might not colonize in mice's gut,but can significantly affect the structure of gut microbiota.A bibliographical survey suggested that there were as few as 8 opportunistic infection cases from 1984 to 2022 and that the possibility for L.gasseri to cause infection is relatively low.Therefore,this work provides a basis for the foods or drugs application of L.gasseri HMV18 and gives a map of experiments for the safety assessment of probiotics.

外文关键词：

ProbioticsLactobacillus gasseriSafety assessmentGut microbiota

作者：

Xianxian Jia、Miaomiao Jia、Xiang Gao、Xiang Li、Mengyuan Wang、Shengqiang Du、Rui Huang、Xiaotong Li、Jun Zhang、Shujin Li、Chunling Ma、Yan Zhang、Bin Cong

展开 >

作者单位：

Department of Pathogen Biology,Institute of basic Medicine,Hebei Medical University,Shijiazhuang 050017,China

Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology,Chinese Academy of Medical Sciences,Shijiazhuang 050017,China

Institute of Basic Medicine,Hebei Medical University,Shijiazhuang 050017,China

College of Forensic Medicine,Hebei Key Laboratory of Forensic Medicine,Collaborative Innovation Center of Forensic Medical Molecular Identification,Hebei Medical University,Shijiazhuang 050017,China

Hebei Food Safety Key Laboratory,Hebei Food Inspection and Research Institute,Shijiazhuang 050227,China

展开 >

基金：

postdoctoral funding of Hebei Medical University,Hebei Province Postdoctoral Research Project Funding河北省自然科学基金CAMS Innovation Found for Medical Sciences2023 Scientific Research Projects of Colleges and Universities in Hebei ProvinceS & T Program of HebeiUndergraduate Innovation Experiment Project from Hebei Medical UniversitySpring rain project of Hebei Medical University

项目编号：

B2022003035H20202065792019-I2M-5-055QN202313118277743DUSIP2019008CYCZ201906

出版年：

2024

DOI：

10.26599/FSHW.2022.9250052

食品科学与人类健康(英文)

CSTPCD

ISSN：

年,卷(期)：2024.13(2)

参考文献量44