首页|Antibacterial mechanism of kojic acid and tea polyphenols against Escherichia coli O157∶H7 through transcriptomic analysis
Antibacterial mechanism of kojic acid and tea polyphenols against Escherichia coli O157∶H7 through transcriptomic analysis
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Escherichia coli O157:H7 is one of the major foodbome pathogenic bacterial that cause infectious diseases in humans.The previous found that a combination of kojic acid and tea polyphenols exhibited better activity against E.coli O157∶H7 than using either alone.This study aimed to explore responses underlying the antibacterial mechanisms of kojic acid and tea polyphenols from the gene level.The functional enrichment analysis by comparing kojic acid and tea polyphenols individually or synergistically against E.coli O157∶H7 found that acid resistance systems in kojic acid were activated,and the cell membrane and genomic DNA were destructed in the cells,resulting in"oxygen starvation".The oxidative stress response triggered by tea polyphenols inhibited both sulfur uptake and the synthesis of ATP,which affected the bacteria's life metabolic process.Interestingly,we found that kojic acid combined with tea polyphenols hindered the uptake of iron that played an essential role in the synthesis of DNA,respiration,tricarboxylic acid cycle.The results suggested that the iron uptake pathways may represent a novel approach for kojic acid and tea polyphenols synergistically against E.coli O157∶H7 and provided a theoretical basis for bacterial pathogen control in the food industry.
School of Food Sciences and Engineering,South China University of Technology,Guangzhou 510640,China
Sericulture & Agri-food Research Institute Guangdong Academy of Agricultural Sciences,Key Laboratory of Functional Foods,Ministry of Agriculture and Rural Affairs,Guangdong Key Laboratory of Agricultural Products Processing,Guangzhou 510610,China
Research Center of Food Safety and Detection,College of Food Science and Engineering,South China University of Technology,Guangzhou 510640,China
国家自然科学基金Research and Development Projects in Key Areas of Guangdong Province广州市科技计划Guangdong key research and development programFoshan and agricultural academy cooperation project and Guangdong Modern Agriculture projectAquatic Products Center Project of GAASGuangzhou Gene Denovo Biotechnology Co.,Ltd.,China
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