Abstract
A ratiometric fluorescent probe for hypoxanthine(Hx)detection was established based on the mimic enzyme and fluorescence characteristics of cobalt-doped graphite-phase carbon nitride(Co doped g-C3N4).In addition to emitting strong fluorescence,the peroxidase activity of Co doped g-C3N4 can catalyze the reaction of O-phenylenediamine and H202 to produce diallyl phthalate which can emit yellow fluorescence at 570 nm.Through the decomposition of Hx by xanthine oxidase,Hx can be indirectly detected by the generating hydrogen peroxide based on the measurement of fluorescent ratio I(F570/F370).The linear range was 1.7-272.2 mg/kg(R2=0.997),and the detection limit was 1.52 mg/kg(3σ/K,n=9).The established method was applied to Hx detection in bass,grass carp,and shrimp,and the data were verified by HPLC.The result shows that the established probe is sensitive,accurate,and reliable,and can be used for Hx detection in aquatic products.
基金项目
国家自然科学基金(21804050)
国家重点研发计划(2018YFD0901003)
Science and Technology Planning Project of Xiamen,China(3502Z20183031)
Fujian Provincial Fund Project(2018J01432)
维普
万方数据