One stone two birds:electrochemical and colorimetric dual-mode biosensor based on copper peroxide/covalent organic framework nanocomposite for ultrasensentive norovirus detection

Guobao Ning ¹Quanmei Duan ¹Huan Liang ¹Huifang Liu ²Min Zhou ¹Chunlan Chen ¹Chong Zhang ³Hui Zhao ²Canpeng Li¹

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作者信息

1. School of Chemical Science and Technology,Yunnan University,Kunming 650091,China
2. Laboratory for Conservation and Utilization of Bio-resource,Yunnan University,Kunming 650091,China
3. Kunming Customs Technology Center,Kunming 650228,China
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Abstract

Norovirus(NoV)is regarded as one of the most common causes of foodborne diarrhea in the world.It is urgent to identify the pathogenic microorganism of the diarrhea in short time.In this work,we developed an electrochemical and colorimetric dual-mode detection for NoV based on the excellent dual catalytic properties of copper peroxide/COF-NH2 nanocomposite(CuO2@COF-NH2).For the colorimetric detection,NoV can be directly detected by the naked eye based on CuO2@COF-NH2 as a laccase-like nonazyme using"peptide-NoV-antibody"recognition mode.The colorimetric assay displayed a wide and quality linear detection range from 1 copy/mL to 5 000 copies/mL of NoV with a low limit of detection(LOD)of 0.125 copy/mL.For the electrochemical detection of NoV,CuO2@COF-NH2 showed an oxidation peak of copper ion from Cu+to Cu2+using"peptide-NoV-antibody"recognition mode.The electrochemical assay showed a linear detection range was 1-5 000 copies/mL with a LOD of 0.152 copy/mL.It's worthy to note that this assay does not need other electrical signal molecule,which provide the stable and sensitive electrochemial detection for NoV.The electrochemical and colorimetric dual-mode detection was used to detect NoV in foods and faceal samples,which has the potential for improving food safety and diagnosing of NoV-infected diarrhea.

Key words

Norovirus/Specific peptides/Electrochemical and colorimetric assay/Dual-mode/Copper peroxide/covalent organic framework

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基金项目

国家重点研发计划(2022YFC2601604)

Major Science and Technology Project of Yunnan Province(202202AE090085)

国家自然科学基金(32160597)

国家自然科学基金(32160236)

Science and technology talent and platform plan of Yunnan()

Key Scientific and Technology Project of Yunnan(202203AC100010)

Spring City Plan:the High-level Talent Promotion and Training Project of Kunming(2022SCP001)

second phase of"Double-First Class"program construction of Yunnan University()

State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Yunnan University(2021KF005)

Key Scientific and Technology Project of Yunnan(202002AE320005)

Program for Excellent Young Talents of Yunnan University()

Program for Donglu Scholars of Yunnan University()

出版年

2024

食品科学与人类健康(英文)

CSTPCDCSCD

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参考文献量47

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