首页|One stone two birds:electrochemical and colorimetric dual-mode biosensor based on copper peroxide/covalent organic framework nanocomposite for ultrasensentive norovirus detection

One stone two birds:electrochemical and colorimetric dual-mode biosensor based on copper peroxide/covalent organic framework nanocomposite for ultrasensentive norovirus detection

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Norovirus(NoV)is regarded as one of the most common causes of foodborne diarrhea in the world.It is urgent to identify the pathogenic microorganism of the diarrhea in short time.In this work,we developed an electrochemical and colorimetric dual-mode detection for NoV based on the excellent dual catalytic properties of copper peroxide/COF-NH2 nanocomposite(CuO2@COF-NH2).For the colorimetric detection,NoV can be directly detected by the naked eye based on CuO2@COF-NH2 as a laccase-like nonazyme using"peptide-NoV-antibody"recognition mode.The colorimetric assay displayed a wide and quality linear detection range from 1 copy/mL to 5 000 copies/mL of NoV with a low limit of detection(LOD)of 0.125 copy/mL.For the electrochemical detection of NoV,CuO2@COF-NH2 showed an oxidation peak of copper ion from Cu+to Cu2+using"peptide-NoV-antibody"recognition mode.The electrochemical assay showed a linear detection range was 1-5 000 copies/mL with a LOD of 0.152 copy/mL.It's worthy to note that this assay does not need other electrical signal molecule,which provide the stable and sensitive electrochemial detection for NoV.The electrochemical and colorimetric dual-mode detection was used to detect NoV in foods and faceal samples,which has the potential for improving food safety and diagnosing of NoV-infected diarrhea.

NorovirusSpecific peptidesElectrochemical and colorimetric assayDual-modeCopper peroxide/covalent organic framework

Guobao Ning、Quanmei Duan、Huan Liang、Huifang Liu、Min Zhou、Chunlan Chen、Chong Zhang、Hui Zhao、Canpeng Li

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School of Chemical Science and Technology,Yunnan University,Kunming 650091,China

Laboratory for Conservation and Utilization of Bio-resource,Yunnan University,Kunming 650091,China

Kunming Customs Technology Center,Kunming 650228,China

国家重点研发计划Major Science and Technology Project of Yunnan Province国家自然科学基金国家自然科学基金Science and technology talent and platform plan of YunnanKey Scientific and Technology Project of YunnanSpring City Plan:the High-level Talent Promotion and Training Project of Kunmingsecond phase of"Double-First Class"program construction of Yunnan UniversityState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Yunnan UniversityKey Scientific and Technology Project of YunnanProgram for Excellent Young Talents of Yunnan UniversityProgram for Donglu Scholars of Yunnan University

2022YFC2601604202202AE0900853216059732160236202203AC1000102022SCP0012021KF005202002AE320005

2024

10.26599/FSHW.2022.9250079

食品科学与人类健康(英文)

食品科学与人类健康(英文)

CSTPCD
ISSN：
年,卷(期)：2024.13(2)
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