Tb3+-nucleic acid probe-based label-free and rapid detection of mercury pollution in food

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外文摘要：Mercury is a threatening pollutant in food,herein,we developed a Tb3+-nucleic acid probe-based label-free assay for mix-and-read,rapid detection of mercury pollution.The assay utilized the feature of light-up fluorescence of terbium ions(Tb3+)via binding with single-strand DNA.Mercury ion,Hg2+induced thymine(T)-rich DNA strand to form a double-strand structure(T-Hg2+-T),thus leading to fluorescence reduction.Based on the principle,Hg2+can be quantified based on the fluorescence of Tb3+,the limit of detection was 0.068 9 μmol/L and the linear range was 0.1-6.0 μmol/L.Due to the specificity of T-Hg2+-T artificial base pair,the assay could distinguish Hg2+from other metal ions.The recovery rate was ranged in 98.71％-101.34％for detecting mercury pollution in three food samples.The assay is low-cost,separation-free and mix-to-read,thus was a competitive tool for detection of mercury pollution to ensure food safety.

外文关键词：

Mercury pollutionFood safetyNucleic acid probeLabel-freeTerbium

作者：

Xuhan Xia、Chenxi Zhou、Yulin Zhu、Yi Dong、Qiang He、Mohammad Rizwan Khan、Yuanlong Chi、Rosa Busquets、Ruijie Deng、Yao Ren

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作者单位：

College of Biomass Science and Engineering,Healthy Food Evaluation Research Center,Sichuan University,Chengdu 610065,China

Department of Chemistry,College of Science,King Saud University,Riyadh 11451,Saudi Arabia

School of Life Sciences,Pharmacy and Chemistry,Kingston University London,Kingston Upon Thames KT1 2EE,UK

基金：

国家自然科学基金Young Elite Scientist Sponsorship Program by CASTResearchers Supporting ProjectKing Saud University,Riyadh,Saudi ArabiaTechnological Innovation Research and Development Project of Chengdu CitySichuan University-Panzhihua City joint Project

项目编号：

22074100YESS20200036RSP-2021/1382019-YF05-317 02266-SN2020CDPZH-5

出版年：

2024

DOI：

10.26599/FSHW.2022.9250085

食品科学与人类健康(英文)

CSTPCD

ISSN：

年,卷(期)：2024.13(2)

参考文献量38