A core-satellite self-assembled SERS aptasensor containing a"biological-silent region"Raman tag for the accurate and ultrasensitive detection of histamine

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外文摘要：Herein,a novel interference-free surface-enhanced Raman spectroscopy(SERS)strategy based on magnetic nanoparticles(MNPs)and aptamer-driven assemblies was proposed for the ultrasensitive detection of histamine.A core-satellite SERS aptasensor was constructed by combining aptamer-decorated Fe3O4@Au MNPs(as the recognize probe for histamine)and complementary DNA-modified silver nanoparticles carrying 4-mercaptobenzonitrile(4-MBN)(Ag@4-MBN@Ag-c-DNA)as the SERS signal probe for the indirect detection of histamine.Under an applied magnetic field in the absence of histamine,the assembly gave an intense Raman signal at"Raman biological-silent"region due to 4-MBN.In the presence of histamine,the Ag@4-MBN@Ag-c-DNA SERS-tag was released from the Fe3O4@Au MNPs,thus decreasing the SERS signal.Under optimal conditions,an ultra-low limit of detection of 0.65 × 10-3 ng/mL and a linear range 10 2-105 ng/mL on the SERS aptasensor were obtained.The histamine content in four food samples were analyzed using the SERS aptasensor,with the results consistent with those determined by high performance liquid chromatography.The present work highlights the merits of indirect strategies for the ultrasensitive and highly selective SERS detection of small biological molecules in complex matrices.

外文关键词：

Surface-enhanced Raman spectroscopyRaman biological-silent regionAptamerHistamine detectionUniversal SERS-tag

作者：

Chen Chen、Yingfang Zhang、Ximo Wang、Xuguang Qiao、Geoffrey I.N.Waterhouse、Zhixiang Xu

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作者单位：

Key Laboratory of Food Processing Technology and Quality Control in Shandong Province,College of Food Science and Engineering,Shandong Agricultural University,Tai'an 271018,China

School of Chemical Sciences,The University of Auckland,Auckland 1142,New Zealand

基金：

国家自然科学基金MacDiarmid Institute for Advanced Materials and NanotechnologyDodd-Walls Centre for Photonic and Quantum Technologies

项目编号：

31972149

出版年：

2024

DOI：

10.26599/FSHW.2022.9250089

食品科学与人类健康(英文)

CSTPCD

ISSN：

年,卷(期)：2024.13(2)

参考文献量34