Abstract
Epicatechin(EC)was used in this study to antagonize the cognitive dysfunction caused by lead(Pb)exposure in mice.Eight-week-old male Kunming mice were treated with PbCl2(20 mg/kg)and/or EC(50 mg/kg)by gavage administration for 4 weeks.Morris water maze test showed that EC could improve memory dysfunction induced by Pb.EC antagonized Ca2+ overload,activated Nrf2 signaling pathway and reduced the accumulation of Pb in the brain and serum,which suggested that EC might alter Pb distribution in mice.In vitro,spectroscopic analysis,potentiometric titration and docking studies were applied to inquiry into the interaction between bovine serum albumin(BSA)and Pb2+in presence or absence of EC.EC was proved to chelate Pb2+and reduced the interaction between BSA and Pb2+.In summary,EC might protect Pb-induced cognitive impairment by activating Nrf2 signaling pathway,and suppressing Pb accumulation via interference on the binding of Pb to albumin.
基金项目
国家重点研发计划(2022YFF1102800)
Graduate Scientific Research Innovation Project of Tianji(2022SKY109)
Project of Tianjin Science and Technology Program(22JCYBJC00360)
Project of Tianjin Science and Technology Program(21ZYJDJC00060)
维普
万方数据