首页|Targeted screening of an anti-inflammatory polypeptide from Rhopilema esculentum Kishinouye cnidoblasts and elucidation of its mechanism in alleviating ulcerative colitis based on an analysis of the gut microbiota and metabolites

Targeted screening of an anti-inflammatory polypeptide from Rhopilema esculentum Kishinouye cnidoblasts and elucidation of its mechanism in alleviating ulcerative colitis based on an analysis of the gut microbiota and metabolites

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Ulcerative colitis(UC)is a recurrent inflammatory bowel disease that imposes a severe burden on families and society.In recent years,exploiting the potential of marine bioactive peptides for the treatment of diseases has become a topic of intense research interest.This study revealed the mechanism underlying the protective effect of the dominant polypeptide PKKVV(Pro-Lys-Lys-Val-Val)of Rhopilema esculentum cnidoblasts against DSS-induced UC through a combined analysis of the metagenome and serum metabolome.Specifically,the polypeptide composition of R.esculentum cnidoblasts was determined by matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF/TOF-MS).Molecular docking showed that the dominant peptide PKKVV could bind better with tumor necrosis factor-α(TNF-α)than the original ligand.Subsequent animal experiments suggested that PKKVV could modulate disorganized gut microorganisms in mice with UC;affect serum metabolites through the arachidonic acid,glycerophospholipid and linoleic acid metabolism pathways;and further alleviate UC symptoms.This study provides a reference for the comprehensive development of marine bioactive substances and nonpharmaceutical treatments for UC.

Ulcerative colitisRhopilema esculentum KishinouyeCnidoblastsMarine bioactive polypeptidesMetagenomeSerum metabolome

Ziyan Wang、Qiuyue Shi、Ying Feng、Jiaojiao Han、Chenyang Lu、Jun Zhou、Zhonghua Wang、Xiurong Su

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State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Ningbo University,Ningbo 315000,China

School of Marine Science,Ningbo University,Ningbo 315000,China

Key Laboratory of Aquaculture Biotechnology(Ningbo University),Ministry of Education,Ningbo University,Ningbo 315000,China

College of Life Sciences,Tonghua Normal University,Tonghua 134000,China

Shandong Beiyou Biotechnology Co.,Ltd.,Weifang 261000,China

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国家重点研发计划浙江省自然科学基金Fund of State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-productsFundamental Research Funds for the Provincial Universities of ZhejiangK.C.Wong Magna Fund of Ningbo University

2018YFD0901102LQ22D060002ZS20190105SJLY2021015

2024

10.26599/FSHW.2022.9250112

食品科学与人类健康(英文)

食品科学与人类健康(英文)

CSTPCD
ISSN：
年,卷(期)：2024.13(3)
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