首页|Punicalagin prevents obesity-related cardiac dysfunction through promoting DNA demethylation in mice

Punicalagin prevents obesity-related cardiac dysfunction through promoting DNA demethylation in mice

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The aim of this study was to investigate whether punicalagin(PU)could prevent obesity-related cardiac dysfunction by promoting DNA demethylation,and to explore its possible mechanism.C57BL/6J mice were fed with standard diet,high-fat diet(HFD),HFD supplemented with resveratrol,low-dose PU(LPU)and high-dose PU(HPU)for 8 weeks.Compared with HFD group,body weight was significantly lower in PU treatment groups,number of cardiomyocytes and the protein level of myosin heavy chain 7B were significantly higher in PU treatment groups.Levels of 5-hydroxymethylcytosine and 5-formylcytosine were significantly lower in HFD group than in other groups.Compared with the HFD group,the protein level of ten-eleven translocation enzyme(TET)2 was significantly higher in PU treatment groups,p-AMP-activated protein kinase(AMPK)was significantly higher in LPU group.Levels of total antioxidant capacity and the protein levels of complexes Ⅱ/Ⅲ/Ⅴ,oxoglutarate dehydrogenase,succinate dehydrogenase B and fumarate hydrolase were significantly lower in HFD group than PU treatment group.The ratio of(succinic acid+fumaric acid)/α-ketoglutarate was significantly higher in HFD group than other groups.In conclusion,PU up-regulated TETs enzyme activities and TET2 protein stability through alleviating mitochondrial dysfunction and activating AMPK,so as to promote DNA demethylation,thus preventing obesity-related cardiac dysfunction.

DNA demethylationMitochondrial functionObesity-related cardiac dysfunctionPunicalaginTen-eleven translocation family enzymes

Shengjie Pei、Run Liu、Qingqing Ma、Peng Jiang、Xin He、Zhongshi Qi、Jiacheng Fang、Xu Yang、Zirui Yao、Xiaoqian Liu、Xianfeng Jing、Lei Chen、Duo Li

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Institute of Nutrition & Health,Qingdao University,Qingdao 266071,China

School of Public Health,Qingdao University,Qingdao 266071,China

Central Laboratory,Guizhou Aerospace Hospital,Zunyi 563000,China

Health Centers of Licha Town,Qingdao 266071,China

College of Computer Science and Technology,Qingdao University,Qingdao 266071,China

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山东省自然科学基金山东省自然科学基金

ZR2020QH294ZR2021QH342

2024

10.26599/FSHW.2022.9250123

食品科学与人类健康(英文)

食品科学与人类健康(英文)

CSTPCD
ISSN：
年,卷(期)：2024.13(3)
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