首页|Calcium-chelating peptides from rabbit bone collagen:characterization,identification and mechanism elucidation

Calcium-chelating peptides from rabbit bone collagen:characterization,identification and mechanism elucidation

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This study aimed to characterize and identify calcium-chelating peptides from rabbit bone collagen and explore the underlying chelating mechanism.Collagen peptides and calcium were extracted from rabbit bone by instant ejection steam explosion(ICSE)combined with enzymatic hydrolysis,followed by chelation reaction to prepare rabbit bone peptide-calcium chelate(RBCP-Ca).The chelating sites were further analyzed by liquid chromatography-tandem mass(LC-MS/MS)spectrometry while the chelating mechanism and binding modes were investigated.The structural characterization revealed that RBCP successfully chelated with calcium ions.Furthermore,LC-MS/MS analysis indicated that the binding sites included both acidic amino acids(Asp and Glu)and basic amino acids(Lys and Arg).Interestingly,three binding modes,namely Inter-Linking,Loop-Linking and Mono-Linking were for the first time found,while Inter-Linking mode accounted for the highest proportion(75.1％),suggesting that chelation of calcium ions frequently occurred between two peptides.Overall,this study provides a theoretical basis for the elucidation of chelation mechanism of calcium-chelating peptides.

Rabbit boneCollagen peptidePeptide-calcium chelateChelation mechanismLiquid chromatography-tandem mass(LC-MS/MS)

Fuhuan Yuan、Yu Fu、Liang Ma、Hankun Zhu、Yong Yu、Xin Feng、Yi Sun、Hongjie Dai、Xin Liu、Zhengfang Liu、Yuhao Zhang

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College of Food Science,Southwest University,Chongqing 400715,China

Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing,Chongqing 400715,China

Key Laboratory of Luminescence Analysis and Molecular Sensing(Southwest University),Ministry of Education,Chongqing 400715,China

Angel Yeast Co.,Ltd.,Yichang 443003,China

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国家重点研发计划国家自然科学基金国家自然科学基金Special key project of Chongqing technology innovation and application developmentChongqing Technology Innovation and Application Development Special Project

2021YFD210010053197210232101980cstc2021jscx-cylhX0014cstc2021jscx-tpyzxX0014

2024

10.26599/FSHW.2022.9250125

食品科学与人类健康(英文)

食品科学与人类健康(英文)

CSTPCD
ISSN：
年,卷(期)：2024.13(3)
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