首页|Stability and transepithelial transport of oligopeptide(KRQKYD)with hepatocyte-protective activity from Jinhua ham in human intestinal Caco-2 monolayer cells

Stability and transepithelial transport of oligopeptide(KRQKYD)with hepatocyte-protective activity from Jinhua ham in human intestinal Caco-2 monolayer cells

扫码查看
原文链接
  • 万方数据
  • 维普
The study evaluated the stability of an oligopeptide(Lys-Arg-Gln-Lys-Tyr-Asp,KRQKYD)and its transport mechanism by simulating gastrointestinal digestion and a model of human intestinal Caco-2 monolayer cells in vitro.In this study,the effects of environmental factors(temperature,pH and NaCl concentration)and simulated gastrointestinal digestion on the stability of KRQKYD were evaluated by indicators of the levels of alanine transaminase(ALT),aspartate transaminase(AST)and malondialdehyde(MDA)in an alcohol-induced hepatocyte injury model.The results showed that KRQKYD still maintained satisfactory hepatocyte-protective activity after treatment with different temperatures(20-80 ℃),pH(3.0-9.0),NaCl concentration(1％-7％)and simulated gastrointestinal digestion,which indicated that KRQKYD showed good stability to environmental factors and simulated gastrointestinal digestion.Furthermore,the intact KRQKYD could be absorbed in a model of Caco-2 monolayer cells with a Papp value of(9.70±0.53)× 10-7 cm/s.Pretreatment with an energy inhibitor(sodium azide),a competitive peptide transporter inhibitor(Gly-Pro)and a transcytosis inhibitor wortmannin did not decrease the level of transepithelial KRQKYD transport,indicating that the transport mechanism of KRQKYD was not associated with energy dependent,vector mediated and endocytosis.The tight junction disruptor cytochalasin D significantly increased the level of transepithelial KRQKYD transport(P＜0.05),suggesting that intact KRQKYD was absorbed by paracellular transport.

Jinhua hamKRQKYD(Lys-Arg-Gln-Lys-Tyr-Asp)Environmental stabilityGastrointestinal digestiveTransport mechanism

Wen Nie、Feiran Xu、Kai Zhou、Jieying Deng、Ying Wang、Baocai Xu

展开 >

Engineering Research Center of Bio-process,Ministry of Education,School of Food and Biological Engineering,Hefei University of Technology,Hefei 230601,China

School of Biological and Food Engineering,Chuzhou University,Chuzhou 239001,China

Major special project of Anhui ProvinceMajor Science and Technology Project of Anhui Province安徽省自然科学基金Special Fund for Anhui Province Agricultural Products Processing Industry Technology System

2021d06050001201903b060200042308085QC115340000211260001000420

2024

10.26599/FSHW.2022.9250127

食品科学与人类健康(英文)

食品科学与人类健康(英文)

CSTPCD
ISSN：
年,卷(期)：2024.13(3)
  • 31