首页|Structural insights on anti-biofilm mechanism of heated slightly acidic electrolyzed water technology against multi-resistant Staphylococcus aureus biofilm on food contact surface
Structural insights on anti-biofilm mechanism of heated slightly acidic electrolyzed water technology against multi-resistant Staphylococcus aureus biofilm on food contact surface
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Slightly acidic electrolyzed water(SAEW)has proven to be an efficient and novel sanitizer in food and agriculture field.This study assessed the efficacy of SAEW(30 mg/L)at 40 ℃ on the inactivation of foodborne pathogens and detachment of multi-resistant Staphylococcus aureus(MRSA)biofilm.Furthermore,the underlying mechanism of MRSA biofilm under heated SAEW at 40 ℃ treatment on metabolic profiles was investigated.The results showed that the heated SAEW at 40 ℃ significantly effectively against foodborne pathogens of 1.96-7.56(lg(CFU/g))reduction in pork,chicken,spinach,and lettuce.The heated SAEW at 40 ℃ treatment significantly reduced MRSA biofilm cells by 2.41(lg(CFU/cm2)).The synergistic effect of SAEW treatment showed intense anti-biofilm activity in decreasing cell density and impairing biofilm cell membranes.Global metabolic response of MRSA biofilms,treated by SAEW at 40 ℃,revealed the alterations of intracellular metabolites,including amino acids,organic acid,fatty acid,and lipid.Moreover,signaling pathways involved in amino acid metabolism,energy metabolism,nucleotide synthesis,carbohydrate metabolites,and lipid biosynthesis were functionally disrupted by the SAEW at 40 ℃ treatment.As per our knowledge,this is the first research to uncover the potential mechanism of heated SAEW treatment against MRSA biofilm on food contact surface.
Multi-resistant Staphylococcus aureusMetabolic profileSAEWBiofilmHurdle technologyElectrode material
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