Bio-screening and quantification of methyl paraben in vinegar and coconut juice separated by HPTLC

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外文摘要：As a widely used food preservative,methyl paraben was experimentally evidenced with serious hormone-like adverse effects.Herein,a high performance thin-layer chromatography platformed bioluminescent bioautography and image analysis for the selective quantification and confirmation of methyl paraben was proposed and validated in vinegar and coconut juice.First,the detectability of the bioautography to the analyte on different layer materials was estimated,revealing that normal silica gel was the best choice.After that,the liquid of sample extract and working solution were separated to overcome the background noises due to co-extracted matrices.The separation result was then coupled to the optimized bioautography,enabling instant and straightforward screening of the targeted compound.For accurate quantification,bioluminescent inhibition pattern caused by the analyte was processed by image analysis,giving useful sensitivity(LOD＞16 mg/kg),precision(RSD＜10.1％)and accuracy(spike-recovery rate 76.9％-112.2％).Finally,the suspected result was confirmed by determining its MS fingerprint,further strengthening the reliability of screening.

外文关键词：

High performance thin-layer chromatographyBio-screeningBioluminescenceImage analysisMethyl paraben

作者：

Yisheng Chen、Xingjun Xi

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作者单位：

College of Food Science and Engineering,Shanxi Agricultural University,Taigu 030801,China

Insitute of Food Nutrition and Safety,Shanxi Agricultural University,Taiyuan 030031,China

Institute of Agricultural Food Standardization,China National Institute of Standardization,Beijing 100191,China

基金：

国家自然科学基金Shanxi Postdoc RewardShanxi Scholarship Council of ChinaShanxi Agricultural University High-Level Talent Project

项目编号：

21804058SXBYKY20220012021-0682021XG013

出版年：

2024

DOI：

10.26599/FSHW.2022.9250135

食品科学与人类健康(英文)

CSTPCD

ISSN：

年,卷(期)：2024.13(3)

参考文献量39