Abstract
In order to improve the bioavailability of lutein(LUT),a novel lutein-stevioside nanoparticle(LUT-STE)were prepared previously,but the information about LUT-STE on protecting of eye health was limited.This study investigated the effect of LUT-STE on antioxidant activity of H2O2-induced human retinal pigment epithelial(ARPE)cells.LUT and LUT-STE(final concentration of 5 μg/mL)significantly enhanced cell viability from(74.84±5.10)％to(81.92±10.01)％(LUT)and(89.33±4.34)％(LUT-STE),and inhibited the cell apoptosis(P＜0.05).After pretreatment with LUT-STE in ARPE cells,the levels of superoxide dismutase(SOD),catalase(CAT)and glutathion peroxidase(GSH-Px)in ARPE cells were significantly increased(P＜0.05),the contents of reactive oxygen species(ROS)and malondialdehyde(MDA)were decreased.In addition,the vascular endothelial growth factor(VEGF)levels were inhibited by 13.61％and 17.39％,respectively,pretreatment with LUT and LUT-STE.Western blotting results showed that the pretreatment with LUT-STE inhibited the expression of caspase-9 and caspase-3 and up-regulated Bcl-2/Bax pathway to inhibit H2O2-induced apoptosis.In summary,the novel delivery LUT-STE had more pronounced inhibitory effect on H2O2-induced damage in human ARPE cells.
基金项目
国家自然科学基金(31801541)
Independent Innovation Fund Project of Agricultural Science and Technology in Jiangsu Province(CX223065)
Major Scientific and Technological Achievements Transformation project of Taizhou(SCG 202105)
Taizhou Science and Technology Support Plan(TN202106)
万方数据