Exploration of cyclooxygenase-2 inhibitory peptides from walnut dreg proteins based on in silico and in vitro analysis

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外文摘要：Walnut dreg protein hydrolysates(WDPHs)exhibit a variety of biological activities,however,the cyclooxygenase-2(COX-2)inhibitory peptide of WDPHs remain unclear.The aim of this study was to rapidly screen for such peptides in WDPHs through a combination of in silico and in vitro analysis.In total,1 262 peptide sequences were observed by nano liquid chromatography/tandem mass spectrometry(nano LC-MS/MS)and 4 novel COX-2 inhibitory peptides(AGFP,FPGA,LFPD,and VGFP)were identified.Enzyme kinetic data indicated that AGFP,FPGA,and LFPD displayed mixed-type COX-2 inhibition,whereas VGFP was a non-competitive inhibitor.This is mainly because the peptides form hydrogen bonds and hydrophobic interactions with residues in the COX-2 active site.These results demonstrate that computer analysis combined with in vitro evaluation allows for rapid screening of COX-2 inhibitory peptides in walnut protein dregs.

外文关键词：

Walnut dreg proteinsCyclooxygenase-2 inhibitory peptideIdentificationVirtual screeningMolecular docking

作者：

Zishan Hong、Jing Xie、Liang Tao、Jing-Jing Dai、Tingting Li、Li Zhang、Yuying Bai、Xia Hu、Jinlian Chen、Jun Sheng、Yang Tian

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作者单位：

College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China

Yunnan Provincial Key Laboratory of Precision Nutrition and Personalized Food Manufacturing,Yunnan Agricultural University,Kunming 650201,China

Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources,Ministry of Education,Yunnan Agricultural University,Kunming 650201,China

School of Tea and Coffee,Pu'er University,Pu'er 665000,China

Yunnan Provincial Engineering Research Center for Edible and Medicinal Homologous Functional Food,Yunnan Agricultural University,Kunming 650201,China

Key Laboratory of Pu-er Tea Science,Ministry of Education,Yunnan Agricultural University,Kunming 650201,China

Yunnan Provincial Engineering Research Center

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基金：

Major Project of Science and Technology Department of Yunnan ProvinceMajor Project of Science and Technology Department of Yunnan ProvinceProject of Yunnan Province Food and Drug Homologous Resources Functional Food Innovation TeamYEFICRC project of Yunnan provincial key programsYunnan Province Ten Thousand Plan Industrial Technology Talents projectYunnan Provincial Department of Science and Technology Agricultural Joint Special Project

项目编号：

202002A A100005202102AE090027-2A30320230572019ZG009YNWR-CYJS-2020-010202101BD070001-120

出版年：

2024

DOI：

10.26599/FSHW.2022.9250143

食品科学与人类健康(英文)

CSTPCD

ISSN：

年,卷(期)：2024.13(3)

参考文献量50