Exploration of cyclooxygenase-2 inhibitory peptides from walnut dreg proteins based on in silico and in vitro analysis

Zishan Hong ¹Jing Xie ¹Liang Tao ¹Jing-Jing Dai ²Tingting Li ³Li Zhang ⁴Yuying Bai ³Xia Hu ⁴Jinlian Chen ³Jun Sheng ⁵Yang Tian⁶

扫码查看

作者信息

1. College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Yunnan Provincial Key Laboratory of Precision Nutrition and Personalized Food Manufacturing,Yunnan Agricultural University,Kunming 650201,China;Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources,Ministry of Education,Yunnan Agricultural University,Kunming 650201,China
2. Yunnan Provincial Key Laboratory of Precision Nutrition and Personalized Food Manufacturing,Yunnan Agricultural University,Kunming 650201,China;School of Tea and Coffee,Pu'er University,Pu'er 665000,China;Yunnan Provincial Engineering Research Center for Edible and Medicinal Homologous Functional Food,Yunnan Agricultural University,Kunming 650201,China
3. College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Yunnan Provincial Key Laboratory of Precision Nutrition and Personalized Food Manufacturing,Yunnan Agricultural University,Kunming 650201,China;Yunnan Provincial Engineering Research Center for Edible and Medicinal Homologous Functional Food,Yunnan Agricultural University,Kunming 650201,China
4. College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources,Ministry of Education,Yunnan Agricultural University,Kunming 650201,China;Yunnan Provincial Engineering Research Center for Edible and Medicinal Homologous Functional Food,Yunnan Agricultural University,Kunming 650201,China
5. College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Key Laboratory of Pu-er Tea Science,Ministry of Education,Yunnan Agricultural University,Kunming 650201,China
6. College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Yunnan Provincial Key Laboratory of Precision Nutrition and Personalized Food Manufacturing,Yunnan Agricultural University,Kunming 650201,China;Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources,Ministry of Education,Yunnan Agricultural University,Kunming 650201,China;School of Tea and Coffee,Pu'er University,Pu'er 665000,China;Yunnan Provincial Engineering Research Center
折叠

Abstract

Walnut dreg protein hydrolysates(WDPHs)exhibit a variety of biological activities,however,the cyclooxygenase-2(COX-2)inhibitory peptide of WDPHs remain unclear.The aim of this study was to rapidly screen for such peptides in WDPHs through a combination of in silico and in vitro analysis.In total,1 262 peptide sequences were observed by nano liquid chromatography/tandem mass spectrometry(nano LC-MS/MS)and 4 novel COX-2 inhibitory peptides(AGFP,FPGA,LFPD,and VGFP)were identified.Enzyme kinetic data indicated that AGFP,FPGA,and LFPD displayed mixed-type COX-2 inhibition,whereas VGFP was a non-competitive inhibitor.This is mainly because the peptides form hydrogen bonds and hydrophobic interactions with residues in the COX-2 active site.These results demonstrate that computer analysis combined with in vitro evaluation allows for rapid screening of COX-2 inhibitory peptides in walnut protein dregs.

Key words

Walnut dreg proteins/Cyclooxygenase-2 inhibitory peptide/Identification/Virtual screening/Molecular docking

引用本文复制引用

基金项目

Major Project of Science and Technology Department of Yunnan Province(202002A A100005)

Major Project of Science and Technology Department of Yunnan Province(202102AE090027-2)

Project of Yunnan Province Food and Drug Homologous Resources Functional Food Innovation Team(A3032023057)

YEFICRC project of Yunnan provincial key programs(2019ZG009)

Yunnan Province Ten Thousand Plan Industrial Technology Talents project(YNWR-CYJS-2020-010)

Yunnan Provincial Department of Science and Technology Agricultural Joint Special Project(202101BD070001-120)

出版年

2024

食品科学与人类健康(英文)

CSTPCDCSCD

ISSN：

参考文献量50

段落导航