Voluntary wheel running ameliorated the deleterious effects of high-fat diet on glucose metabolism,gut microbiota and microbial-associated metabolites

Ling Zhang ¹Wenyu Zou ¹Yongyan Hu ²Honghua Wu ¹Ying Gao ¹Junqing Zhang ¹Jia Zheng¹

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作者信息

1. Department of Endocrinology,Peking University First Hospital,Beijing 100034,China
2. Laboratory Animal Facility,Peking University First Hospital,Beijing 100034,China
折叠

Abstract

Exercise training is critical for the early prevention and treatment of obesity and diabetes mellitus.However,the mechanism with gut microbiota and fecal metabolites underlying the effects of voluntary wheel running on high-fat diet induced abnormal glucose metabolism has not been fully elaborated.C57BL/6 male mice were randomly assigned to 4 groups according to diets(fed with normal chow diet or high-fat diet)and running paradigm(housed in static cage or with voluntary running wheel).An integrative 16S rDNA sequencing and metabolites profiling was synchronously performed to characterize the effects of voluntary wheel running on gut microbiota and metabolites.It showed that voluntary wheel running prevented the detrimental effects of high-fat feeding on glucose metabolism.16S rDNA sequencing showed remarkable changes in Rikenella and Marvinbryantia genera.Metabolic profiling indicated multiple altered metabolites,which were enriched in secondary bile acid biosynthesis signaling.In conclusion,our study indicated that voluntary wheel running significantly improved glucose metabolism and counteracted the deleterious effects of high-fat feeding on body weight and glucose intolerance.We further found that voluntary wheel running could integratively program gut microbiota composition and fecal metabolites changes,and may regulate muricholic acid metabolism and secondary bile acid biosynthesis in high-fat fed mice.

Key words

High-fat diet/Voluntary wheel running/Gut microbiota/Metabolomics/Glucose metabolism

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基金项目

国家自然科学基金(81800703)

国家自然科学基金(81970701)

北京市科技新星计划(Z201100006820117)

北京市科技新星计划(20220484181)

北京市自然科学基金(7184252)

北京市自然科学基金(7214258)

Fundamental Research Funds for the Central Universities,Peking University Clinical Scientist Training Program(BMU2023PYJH022)

China Endocrine and Metabolism Young Scientific Talent Research Project(2022-N-02-01)

Peking University Medicine Seed Fund for Interdisciplinary Research()

中央高校基本科研业务费专项(BMU2021MX013)

China Diabetes Young Scientific Talent Research Project()

Bethune-Merck Diabetes Research Fund of Bethune Charitable Foundation(G2018030)

出版年

2024

食品科学与人类健康(英文)

CSTPCDCSCD

ISSN：

参考文献量43

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