Andrias davidianus bone peptides alleviates hyperuricemia-induced kidney damage in vitro and in vivo

Wei Li ¹Haihong Chen ²Hongliang Chen ¹Ziyin Li ³Wei Hu ¹Qinxuan Zhou ¹Bing Xu ²Yi Wang ⁴Xinhui Xing⁵

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作者信息

1. Institute of Biopharmaceutical and Health Engineering,Tsinghua Shenzhen International Graduate School,Shenzhen 518055,China
2. Institute of Biopharmaceutical and Health Engineering,Tsinghua Shenzhen International Graduate School,Shenzhen 518055,China;Institute of Biomedical Health Technology and Engineering,Shenzhen Bay Laboratory,Shenzhen 440300,China
3. Affiliated Dongguan Hospital,Southern Medical University(Dongguan People's Hospital),Dongguan 523059,China
4. Key Laboratory for Industrial Biocatalysis,Ministry of Education,Institute of Biochemical Engineering,Department of Chemical Engineering,Beijing 100084,China;Center for Synthetic and Systems Biology,Tsinghua University,Beijing 100084,China
5. Institute of Biopharmaceutical and Health Engineering,Tsinghua Shenzhen International Graduate School,Shenzhen 518055,China;Institute of Biomedical Health Technology and Engineering,Shenzhen Bay Laboratory,Shenzhen 440300,China;Key Laboratory for Industrial Biocatalysis,Ministry of Education,Institute of Biochemical Engineering,Department of Chemical Engineering,Beijing 100084,China;Center for Synthetic and Systems Biology,Tsinghua University,Beijing 100084,China
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Abstract

Hyperuricemia(HUA)is a vital risk factor for chronic kidney diseases(CKD)and development of functional foods capable of protecting CKD is of importance.This paper aimed to explore the amelioration effects and mechanism of Andrias davidianus bone peptides(ADBP)on HUA-induced kidney damage.In the present study,we generated the standard ADBP which contained high hydrophobic amino acid and low molecular peptide contents.In vitro results found that ADBP protected uric acid(UA)-induced HK-2 cells from damage by modulating urate transporters and antioxidant defense.In vivo results indicated that ADBP effectively ameliorated renal injury in HUA-induced CKD mice,evidenced by a remarkable decrease in serum UA,creatinine and blood urea nitrogen,improving kidney UA excretion,antioxidant defense and histological kidney deterioration.Metabolomic analysis highlighted 14 metabolites that could be selected as potential biomarkers and attributed to the amelioration effects of ADBP on CKD mice kidney dysfunction.Intriguingly,ADBP restored the gut microbiome homeostasis in CKD mice,especially with respect to the elevated helpful microbial abundance,and the decreased harmful bacterial abundance.This study demonstrated that ADBP displayed great nephroprotective effects,and has great promise as a food or functional food ingredient for the prevention and treatment of HUA-induced CKD.

Key words

Andrias davidianus bone peptides/Hyperuricemia/Uric acid/Chronic kidney disease/Gut microbiota

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基金项目

Shenzhen Agricultural Development Special Fund(Fishery)Agricultural High-Tech Project([2021]735)

Shenzhen Science and Technology Innovation Commission(KCXFZ20201221173207022)

Youth Science Foundation Project(32101936)

出版年

2024

食品科学与人类健康(英文)

CSTPCD

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