Abstract
Sensitive detection of Staphylococcus aureus enterotoxin B(SEB)is of importance for preventing food poisoning from threatening human health.In this work,an electrochemical and colorimetric dual-signal detection assay of SEB was developed.The probe(Ab2/AuPt@Fe-N-C)was bound to SEB captured by Abl,where the Ab2/AuPt@Fe-N-C triggered methylene blue degradation and resulted in the decrease of electrochemical signal.Furthermore,the probe catalyzed the oxidation of 3,3',5,5'-tetramethyl biphenyl to generate a colorimetric absorbance at 652 nm.Once the target was captured and formed a sandwich-like complex,the color changed from colorless to blue.SEB detection by colorimetric and electrochemical methods showed a linear relationship in the concentration ranges of 0.000 2-10.000 0 and 0.000 5-10.000 0 ng/mL,with limits of detection of 0.066 7 and 0.167 0 pg/mL,respectively.The dual-signal biosensor was successfully used to detect SEB in milk and water samples,which has great potential in toxin detection in food and the environment.
基金项目
Major Science and Technology Project of Yunnan Province(202302AE090022)
Key Research and Development Program of Yunnan(202203AC 100010)
国家自然科学基金(32160597)
国家自然科学基金(32160236)
国家自然科学基金(32371463)
国家重点研发计划(2022YFC2601604)
Cardiovascular Ultrasound Innovation Team of Yunnan Province(202305AS350021)
Spring City Plan:the High-level Talent Promotion and Training Project of Kunming(2022SCP001)
second phase of"Double-First Class"Program Construction of Yunnan University()
维普
万方数据