Electrochemical and colorimetric dual-signal detection of Staphylococcus aureus enterotoxin B based on AuPt bimetallic nanoparticles loaded Fe-N-C single atom nanocomposite

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外文摘要：Sensitive detection of Staphylococcus aureus enterotoxin B(SEB)is of importance for preventing food poisoning from threatening human health.In this work,an electrochemical and colorimetric dual-signal detection assay of SEB was developed.The probe(Ab2/AuPt@Fe-N-C)was bound to SEB captured by Abl,where the Ab2/AuPt@Fe-N-C triggered methylene blue degradation and resulted in the decrease of electrochemical signal.Furthermore,the probe catalyzed the oxidation of 3,3',5,5'-tetramethyl biphenyl to generate a colorimetric absorbance at 652 nm.Once the target was captured and formed a sandwich-like complex,the color changed from colorless to blue.SEB detection by colorimetric and electrochemical methods showed a linear relationship in the concentration ranges of 0.000 2-10.000 0 and 0.000 5-10.000 0 ng/mL,with limits of detection of 0.066 7 and 0.167 0 pg/mL,respectively.The dual-signal biosensor was successfully used to detect SEB in milk and water samples,which has great potential in toxin detection in food and the environment.

外文关键词：

Staphylococcus aureus enterotoxinElectrochemical immunosensorColorimetric assayMOF@borophene compositeDual-functional Fe-N-C signal atom catalyst

作者：

Huan Liang、Hongcheng Liu、Haojian Lin、Guobao Ning、Xiaokang Lu、Siying Ma、Fei Liu、Hui Zhao、Canpeng Li

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作者单位：

School of Chemical Science and Technology,Yunnan University,Key Laboratory of Medicinal Chemistry for Natural Resource,Ministry of Education,Kunming 650091,China

Institute of Quality Standard and Testing Technology,Yunnan Academy of Agricultural Science,Supervision and Testing Center for Farm Product Quality,Ministry of Agriculture,Kunming 650223,China

State Key Laboratory of Optoelectronic Materials and Technologies,Guangdong Province Key Laboratory of Display Material and Technology,School of Electronics and Information Technology,Sun Yat-sen University,Guangzhou 510275,China

Laboratory for Conservation and Utilization of Bio-resource,Yunnan University,Kunming 650091,China

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基金：

Major Science and Technology Project of Yunnan ProvinceKey Research and Development Program of Yunnan国家自然科学基金国家自然科学基金国家自然科学基金国家重点研发计划Cardiovascular Ultrasound Innovation Team of Yunnan ProvinceSpring City Plan:the High-level Talent Promotion and Training Project of Kunmingsecond phase of"Double-First Class"Program Construction of Yunnan University

项目编号：

202302AE090022202203AC 1000103216059732160236323714632022YFC2601604202305AS3500212022SCP001

出版年：

2024

DOI：

10.26599/FSHW.2022.9250168

食品科学与人类健康(英文)

CSTPCD

ISSN：

年,卷(期)：2024.13(4)

参考文献量56