Safety assessment of a novel marine multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 according to phenotype and whole genome-sequencing analysis

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外文摘要：The application of microorganisms as probiotics is limited due to lack of safety evaluation.Here,a novel multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 with aroma-producing properties was identified from marine mangrove microorganisms.Its safety and probiotic properties were assessed in accordance with phenotype and whole-genome sequencing analysis.Results showed that the genes and phenotypic expression of related virulence,antibiotic resistance and retroelement were rarely found.Hyphal morphogenesis genes(SIT4,HOG1,SPA2,ERK1,ICL1,CST20,HSP104,TPS1,and RHO1)and phospholipase secretion gene(VPS4)were annotated.True hyphae and phospholipase were absent.Only one retroelement(Tad1-65_BG)was found.Major biogenic amines(BAs)encoding genes were absent,except for spermidine synthase(JA9_002594),spermine synthase(JA9_004690),and tyrosine decarboxylase(inx).The production of single BAs and total BAs was far below the food-defined thresholds.GXDK6 had no resistance to common antifungal drugs.Virulence enzymes,such as gelatinase,DNase,hemolytic,lecithinase,and thrombin were absent.Acute toxicity test with mice demonstrated that GXDK6 is safe.GXDK6 has a good reproduction ability in the simulation gastrointestinal tract.GXDK6 also has a strong antioxidant ability,β-glucosidase,and inulinase activity.To sum up,GXDK6 is considered as a safe probiotic for human consumption and food fermentation.

外文关键词：

Meyerozyma guilliermondiiSafety assessmentProbioticsMarine mangrove microorganismsWhole-genome sequencing analysis

作者：

Xueyan Mo、Mengcheng Zhou、Yanmei Li、Lili Yu、Huashang Bai、Peihong Shen、Xing Zhou、Haojun Zhu、Huijie Sun、Ru Bu、Chengjian Jiang

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作者单位：

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,Guangxi Research Center for Microbial and Enzyme Engineering Technology,College of Life Science and Technology,Guangxi University,Nanning 530004,China

National Engineering Research Center for Non-Food Biorefinery,State Key Laboratory of Non-Food Biomass and Enzyme Technology,Guangxi Research Center for Biological Science and Technology,Guangxi Academy of Sciences,Nanning 530007,China

Guangxi Key Laboratory for Green Proce

基金：

Funding Project of Chinese Central Government Guiding to the Guangxi Local Science and Technology DevelopmentNatural Science Fund for Distinguished Young Scholars of Guangxi Zhuang Autonomous Region of ChinaFunding Project of Chinese Central Government Guiding to the Nanning Local Science and Technology DevelopmentFunding Projects of Guangxi Key Research and Development PlanFunding Project of Technological Development from Angel Yeast(Chongzuo)Co.,LtdInnovation Project of Guangxi Graduate Education

项目编号：

GUIKEZY211950212019GXNSFFA24501120231012GUIKE AB23075173JS1006020230722019YCBZ2021012

出版年：

2024

DOI：

10.26599/FSHW.2022.9250170

食品科学与人类健康(英文)

CSTPCD

ISSN：

年,卷(期)：2024.13(4)

参考文献量57