Safety assessment of a novel marine multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 according to phenotype and whole genome-sequencing analysis

Xueyan Mo ¹Mengcheng Zhou ¹Yanmei Li ¹Lili Yu ¹Huashang Bai ¹Peihong Shen ¹Xing Zhou ²Haojun Zhu ²Huijie Sun ¹Ru Bu ¹Chengjian Jiang³

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作者信息

1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,Guangxi Research Center for Microbial and Enzyme Engineering Technology,College of Life Science and Technology,Guangxi University,Nanning 530004,China
2. National Engineering Research Center for Non-Food Biorefinery,State Key Laboratory of Non-Food Biomass and Enzyme Technology,Guangxi Research Center for Biological Science and Technology,Guangxi Academy of Sciences,Nanning 530007,China
3. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,Guangxi Research Center for Microbial and Enzyme Engineering Technology,College of Life Science and Technology,Guangxi University,Nanning 530004,China;National Engineering Research Center for Non-Food Biorefinery,State Key Laboratory of Non-Food Biomass and Enzyme Technology,Guangxi Research Center for Biological Science and Technology,Guangxi Academy of Sciences,Nanning 530007,China;Guangxi Key Laboratory for Green Proce
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Abstract

The application of microorganisms as probiotics is limited due to lack of safety evaluation.Here,a novel multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 with aroma-producing properties was identified from marine mangrove microorganisms.Its safety and probiotic properties were assessed in accordance with phenotype and whole-genome sequencing analysis.Results showed that the genes and phenotypic expression of related virulence,antibiotic resistance and retroelement were rarely found.Hyphal morphogenesis genes(SIT4,HOG1,SPA2,ERK1,ICL1,CST20,HSP104,TPS1,and RHO1)and phospholipase secretion gene(VPS4)were annotated.True hyphae and phospholipase were absent.Only one retroelement(Tad1-65_BG)was found.Major biogenic amines(BAs)encoding genes were absent,except for spermidine synthase(JA9_002594),spermine synthase(JA9_004690),and tyrosine decarboxylase(inx).The production of single BAs and total BAs was far below the food-defined thresholds.GXDK6 had no resistance to common antifungal drugs.Virulence enzymes,such as gelatinase,DNase,hemolytic,lecithinase,and thrombin were absent.Acute toxicity test with mice demonstrated that GXDK6 is safe.GXDK6 has a good reproduction ability in the simulation gastrointestinal tract.GXDK6 also has a strong antioxidant ability,β-glucosidase,and inulinase activity.To sum up,GXDK6 is considered as a safe probiotic for human consumption and food fermentation.

Key words

Meyerozyma guilliermondii/Safety assessment/Probiotics/Marine mangrove microorganisms/Whole-genome sequencing analysis

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基金项目

Funding Project of Chinese Central Government Guiding to the Guangxi Local Science and Technology Development(GUIKEZY21195021)

Natural Science Fund for Distinguished Young Scholars of Guangxi Zhuang Autonomous Region of China(2019GXNSFFA245011)

Funding Project of Chinese Central Government Guiding to the Nanning Local Science and Technology Development(20231012)

Funding Projects of Guangxi Key Research and Development Plan(GUIKE AB23075173)

Funding Project of Technological Development from Angel Yeast(Chongzuo)Co.,Ltd(JS1006020230722019)

Innovation Project of Guangxi Graduate Education(YCBZ2021012)

出版年

2024

食品科学与人类健康(英文)

CSTPCD

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参考文献量57

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