Structural elucidation of mulberry leaf oligosaccharide and its selective promotion of gut microbiota to alleviate type 2 diabetes mellitus

Tenggen Hu ¹Yuanshan Yu ²Jijun Wu ³Yujuan Xu ³Gengsheng Xiao ⁴Kejing An ³Erna Li ³Sentai Liao ³Yuxiao Zou³

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作者信息

1. Sericultural & Agri-Food Research Institute,Guangdong Academy of Agricultural ScienceslKey Laboratory of Functional Foods,Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing,Guangzhou 510610,China;Huagongliya(Foshan)Technology Industry Co.,Ltd.,Foshan 528313,China;Heyuan Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Heyuan 517001,China
2. Sericultural & Agri-Food Research Institute,Guangdong Academy of Agricultural ScienceslKey Laboratory of Functional Foods,Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing,Guangzhou 510610,China;Huagongliya(Foshan)Technology Industry Co.,Ltd.,Foshan 528313,China
3. Sericultural & Agri-Food Research Institute,Guangdong Academy of Agricultural ScienceslKey Laboratory of Functional Foods,Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing,Guangzhou 510610,China
4. Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China
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Abstract

Two oligosaccharide fractions(MLO 2-1 and 2-2)were purified from enzymatic hydrolysate of mulberry leaf polysaccharide.The results of simulated digestion showed that MLO 2-2 was a digestible oligosaccharide,which could be degraded by human digestive juice;while MLO 2-1 possessed the non-digestible property in the upper gastrointestinal tract and performed the function by regulating the gut microbiota.Hence,MLO 2-1 was selected for the further analysis.The structure of MLO 2-1 was elucidated as follow:α-T-Glcp-(1→3)-β-Glcp-(1→5)-α-Araf-(1→5)-α-Araf-1→5)-α-Araf-(1 →3)-α-(2-OAc)-Glcp-1.The in vitro fecal fermentation results showed that MLO 2-1 could modulate the composition of gut microbiota.Meanwhile,MLO 2-1 was effectively metabolized by fecal bacteria to produce lactate and short chain fatty acids,especially acetate and butyrate.The specific metabolic pathways of MLO 2-1 by gut microbiota were further illuminated.Gut microbiota analysis revealed that MLO 2-1 selectively promoted the growth of Ligilactobacillus murinus,a commensal bacterium presented a reduced level in T2DM mice.Animal experiments indicated that MLO 2-1 and L.murinus exhibited hypoglycemic activities.These results demonstrated that MLO 2-1 might alleviate T2DM by selectively accelerating the proliferation of L.murinus.

Key words

Mulberry leaf oligosaccharide/Characterization/Gut microbiota/Lactobacillus murinus/Hypoglycemic activity

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基金项目

云南省重点研发计划(202202AE090023)

Key Research and Development Program of Guangdong Province(2022B0202040002)

Key Research and Development Program of Guangdong Province(2022B0202050001)

Key Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food,Ministry of Agriculture()

Heyuan Branch,Guangdong Laboratory for Lingnan Modern Agriculture Project(DT20220026)

Talent Introduction Program of Guangdong Academy of Agricultural Sciences(R2022YJ-YB3004)

Basic and Applied Basic Research Project of Guangdong Province(2022A1515110227)

Basic and Applied Basic Research Project of Guangdong Province(2023A1515012386)

广州市科技计划(2023A04J0828)

Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams(202109TD)

National University Science Park,South China University of Technology(KJYS2021KZ05)

出版年

2024

食品科学与人类健康(英文)

CSTPCDCSCD

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