Structural elucidation of mulberry leaf oligosaccharide and its selective promotion of gut microbiota to alleviate type 2 diabetes mellitus

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外文摘要：Two oligosaccharide fractions(MLO 2-1 and 2-2)were purified from enzymatic hydrolysate of mulberry leaf polysaccharide.The results of simulated digestion showed that MLO 2-2 was a digestible oligosaccharide,which could be degraded by human digestive juice;while MLO 2-1 possessed the non-digestible property in the upper gastrointestinal tract and performed the function by regulating the gut microbiota.Hence,MLO 2-1 was selected for the further analysis.The structure of MLO 2-1 was elucidated as follow:α-T-Glcp-(1→3)-β-Glcp-(1→5)-α-Araf-(1→5)-α-Araf-1→5)-α-Araf-(1 →3)-α-(2-OAc)-Glcp-1.The in vitro fecal fermentation results showed that MLO 2-1 could modulate the composition of gut microbiota.Meanwhile,MLO 2-1 was effectively metabolized by fecal bacteria to produce lactate and short chain fatty acids,especially acetate and butyrate.The specific metabolic pathways of MLO 2-1 by gut microbiota were further illuminated.Gut microbiota analysis revealed that MLO 2-1 selectively promoted the growth of Ligilactobacillus murinus,a commensal bacterium presented a reduced level in T2DM mice.Animal experiments indicated that MLO 2-1 and L.murinus exhibited hypoglycemic activities.These results demonstrated that MLO 2-1 might alleviate T2DM by selectively accelerating the proliferation of L.murinus.

外文关键词：

Mulberry leaf oligosaccharideCharacterizationGut microbiotaLactobacillus murinusHypoglycemic activity

作者：

Tenggen Hu、Yuanshan Yu、Jijun Wu、Yujuan Xu、Gengsheng Xiao、Kejing An、Erna Li、Sentai Liao、Yuxiao Zou

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作者单位：

Sericultural & Agri-Food Research Institute,Guangdong Academy of Agricultural ScienceslKey Laboratory of Functional Foods,Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing,Guangzhou 510610,China

Huagongliya(Foshan)Technology Industry Co.,Ltd.,Foshan 528313,China

Heyuan Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Heyuan 517001,China

Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China

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基金：

云南省重点研发计划Key Research and Development Program of Guangdong ProvinceKey Research and Development Program of Guangdong ProvinceKey Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food,Ministry of AgricultureHeyuan Branch,Guangdong Laboratory for Lingnan Modern Agriculture ProjectTalent Introduction Program of Guangdong Academy of Agricultural SciencesBasic and Applied Basic Research Project of Guangdong ProvinceBasic and Applied Basic Research Project of Guangdong Province广州市科技计划Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation TeamsNational University Science Park,South China University of Technology

项目编号：

202202AE0900232022B02020400022022B0202050001DT20220026R2022YJ-YB30042022A15151102272023A15150123862023A04J0828202109TDKJYS2021KZ05

出版年：

2024

DOI：

10.26599/FSHW.2022.9250180

食品科学与人类健康(英文)

CSTPCD

ISSN：

年,卷(期)：2024.13(4)

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