Analysis of quality-related proteins in golden pompano(Trachinotus ovatus)fillets with modified atmosphere packaging under superchilling storage

Chuang Pan ¹Xiaofan Zhang ²Shengjun Chen ¹Yong Xue ³Yanyan Wu ⁴Yueqi Wang ⁴Di Wang⁴

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作者信息

1. South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Aquatic Product Processing,Ministry of Agriculture and Rural Affairs,National R&D Centre for Aquatic Product Processing,Guangzhou 510300,China;Co-Innovation Center of Jiangsu Marine Bio-industry Technology,Jiangsu Ocean University,Lianyungang 222005,China;Collaborative Innovation Center of Seafood Deep Processing,Dalian Polytechnic University,Dalian 116034,China;Key Laboratory of Efficient Utilization and Proc
2. South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Aquatic Product Processing,Ministry of Agriculture and Rural Affairs,National R&D Centre for Aquatic Product Processing,Guangzhou 510300,China;Co-Innovation Center of Jiangsu Marine Bio-industry Technology,Jiangsu Ocean University,Lianyungang 222005,China;Collaborative Innovation Center of Seafood Deep Processing,Dalian Polytechnic University,Dalian 116034,China;College of Food Science and Engineering,Ocean Un
3. Collaborative Innovation Center of Seafood Deep Processing,Dalian Polytechnic University,Dalian 116034,China
4. South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Aquatic Product Processing,Ministry of Agriculture and Rural Affairs,National R&D Centre for Aquatic Product Processing,Guangzhou 510300,China;Co-Innovation Center of Jiangsu Marine Bio-industry Technology,Jiangsu Ocean University,Lianyungang 222005,China;Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province,Sanya Tropical Fisheries Research Institute,Sanya 57201
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Abstract

Here,we aimed to study the changes in proteome of golden pompano fillets during post-mortem storage.Tandem mass tags(TMT)-labeled quantitative proteomic strategy was applied to investigate the relationships between protein changes and quality characteristics of modified atmosphere packaging(MAP)fillets during superchilling(-3 ℃)storage.Scanning electron microscopy was used to show that the muscle histology microstructure of fillets was damaged to varying degrees,and low-field nuclear magnetic resonance was used to find that the immobilized water and free water in the muscle of fillets changed significantly.Total sulfhydryl content,TCA-soluble peptides and Ca-ATPase activity also showed that the fillet protein had a deterioration by oxidation and denaturation.The Fresh(FS),MAP,and air packaging(AP)groups were set.Total of 150 proteins were identified as differential abundant proteins(DAPs)in MAP/FS,while 209 DAPs were in AP/FS group.The KEGG pathway analysis indicated that most DAPs were involved in binding proteins and protein turnover.Correlation analysis found that 52 DAPs were correlated with quality traits.Among them,8 highly correlated DAPs are expected to be used as potential quality markers for protein oxidation and water-holding capacity.These results provide a further understanding of the muscle deterioration mechanism of packaging golden pompano fillets during superchilling.

Key words

Tandem mass tags(TMT)proteomics/Trachinotus ovatus/Modified atmosphere packaging/Superchilling storage/Low-field nuclear magnetic resonance/Protein deterioration

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基金项目

Central Public-Interest Scientific Institution Basal Research Fund,CAFS(2023TD74)

Central Public-Interest Scientific Institution Basal Research Fund,CAFS(2023TD78)

Earmarked Fund for CARS-47(CARS-47)

Guangdong Provincial Science and Technology Plan Project(2023B0202010015)

Central Public-Interest Scientific Institution Basal Research Fund,CAFS(SKJC-2020-02-013)

Special Funds for Promoting Economic Development in Guangdong Province(2019B14)

出版年

2024

食品科学与人类健康(英文)

CSTPCD

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参考文献量40

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