首页|Puerariae Radix protects against ulcerative colitis in mice by inhibiting NLRP3 inflammasome activation
Puerariae Radix protects against ulcerative colitis in mice by inhibiting NLRP3 inflammasome activation
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Ulcerative colitis(UC)is a common inflammatory disease of the gastrointestinal tract.Traditional Chinese medicine(TCM)has long been used in Asia as a treatment for UC and Puerariae Radix(PR)is a reliable anti-diarrheal therapy.The aims of this study were to investigate the protective effect of PR using the dextran sulfate sodium salt(DSS)-induced UC model in mice and identify molecular mechanisms of PR action.The chemical constituents of PR via ultra-performance liquid chromatography/tandem mass spectrometry and identified potential PR and UC targets using a network pharmacology(NP)approach were obtained to guide mouse experiments.A total of 180 peaks were identified from PR including 48 flavonoids,46 organic acids,14 amino acids,8 phenols,8 carbohydrates,7 alkaloids,6 coumarins and 43 other constituents.NP results showed that caspase-1 was the most dysregulated of the core genes associated with UC.A PR dose of 0.136 mg/g administered to DSS treated mice reversed weight loss and decreased colon lengths found in UC mice.PR also alleviated intestinal mucosal shedding,inflammatory cell infiltration and mucin loss.PR treatment suppressed upregulation of NOD-like receptor protein 3(NLRP3),cysteinyl aspartate-specific proteases-1(caspase-1),apoptosis-associated speck-like(ASC)and gasdermin D(GSDMD)at both the protein and mRNA expression levels.The addition of a small molecule dual-specificity phosphatase inhibitor NSC 95397 inhibited the positive effects of PR.These results indicated that PR exerts a protective effect on DSS-induced colitis by inhibiting NLRP3 inflammasome activation in mice.
Puerariae RadixUlcerative colitisMolecular mechanismsPyroptosisNOD-like receptor protein 3 inflammasome
Innovation Centre of Chinese Veterinary Medicine,College of Veterinary Medicine,China Agricultural University,Beijing 100086,China
National Center of Technology Innovation for Medicinal Function of Food,National Food and Strategic Reserves Administration,Beijing 100193,China
Inner Mongolia Medical University,Hohhot 010059,China
China Agricultural University Yantai Institute,Yantai 264670,China
Shandong Academy of Agricultural Sciences,Jinan 250100,China
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国家自然科学基金Central Significant Changes in the Project at the Corresponding Level(Valuable Resources Capacity-Building for Sustainable UtiliChinese Herbal Medicine Industry Innovation Team of Shandong Province Agricultural Technology System
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