Abstract
Melamine is one of the most frequently detected adulterants in dairy products.The current study proposes a surface-enhanced Raman spectroscopy(SERS)-based analytical tool for fast and reliable screening of melamine in bovine milk.A hand-held Raman spectrometer was used in conjunction with a substrate composed of silver nanoparticles(AgNPs)that was 2D printed onto glass fiber(GF)filter paper.Under optimized conditions,a sensitive and fingerprint-like signal at 674 cm-1 was obtained.The AgNPs/GF substrate exhibited high sensitivity to melamine in milk down to 1.949 8×10-5 mg/mL,which is well below the USA and EU safety limits(2.5×10-3 mg/mL).Remarkably,the proposed technology was also highly reproducible,showing spot-to-spot and block-to-block variations below 3.3%and 4.9%at 674 cm-1 in Raman intensity,respectively.The characteristic peak intensity and concentration of melamine showed an acceptable linear relationship(R2=0.990 9)within the range of 0.000 1-1 mg/mL.Overall,the method established in this study can provide an efficient and effective method for the quantitative target screening and detection of melamine in dairy products.
基金项目
National Natural Science Foundation of China(21804058)
Shanxi Scholarship Council of China(2021-068)
Shanxi Agricultural University High-Level Talent Project(2021XG013)
Shanxi Postdoc Reward(SXBYKY2022001)
维普
万方数据