Dioscin from Polygonatum sibiricum induces apoptosis and autophagy in Ishikawa human endometrial cancer cell and in vivo

Xiaoli Li ¹Runhui Ma ²Zhijing Ni ³Wei Wang ³Kiran Thakur ²Jianguo Zhang ²Zhaojun Wei²

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作者信息

1. School of Biological Science and Engineering,North Minzu University,Yinchuan 750021,China;School of Food and Biological Engineering,Hefei University of Technology,Hefei 230009,China;School of Public Health,Anhui University of Science and Technology,Huainan 232001,China
2. School of Biological Science and Engineering,North Minzu University,Yinchuan 750021,China;School of Food and Biological Engineering,Hefei University of Technology,Hefei 230009,China
3. School of Biological Science and Engineering,North Minzu University,Yinchuan 750021,China
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Abstract

With an aim to comprehend the precise regulatory mechanism of dioscin against endometrial carcinoma(EC),we firstly extracted the components from Polygonatum sibiricum followed by identification and structural characterization.The anti-EC activity of dioscin was initially determined based on the inhibition of Ishikawa cell proliferation and tumor growth.The high-throughput sequencing data indicated that dioscin not only promoted apoptosis,including decrease of poly ADP-ribose polymerase(PARP)and B-cell lymphoma-2(Bcl-2)and increase of c-PARP and Bcl-2-associcated agonist of cell death(Bad),but also induced autophagy,including increase of autophagic lysosomes and LC3II/LC3I ratio.Mechanistic exploration suggested that dioscin induced autophagy and apoptosis through inhibition of PI3K/AKT/mTOR signaling pathway.Besides,the dioscin-regulated p53 pathway was mainly involved in autophagy induction.Furthermore,inhibition of Ishikawa cell autophagy was linked to dioscin-induced apoptosis.Our data suggest the immense potential of dioscin for the development of functional food for EC and related medical application.

Key words

Dioscin/Ishikawa cell/Apoptosis/Autophagy/PI3K/AKT/p53

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基金项目

National Key Research&Development Program of China(2022YFF1100305)

National Natural Science Foundation of Ningxia Province(2021AAC02019)

National Natural Science Foundation of Ningxia Province(2022AAC03230)

Key research and development projects in Ningxia province(2021BEF02013)

出版年

2024

食品科学与人类健康(英文)

CSTPCD

ISSN：

段落导航