首页|Dioscin from Polygonatum sibiricum induces apoptosis and autophagy in Ishikawa human endometrial cancer cell and in vivo

Dioscin from Polygonatum sibiricum induces apoptosis and autophagy in Ishikawa human endometrial cancer cell and in vivo

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With an aim to comprehend the precise regulatory mechanism of dioscin against endometrial carcinoma(EC),we firstly extracted the components from Polygonatum sibiricum followed by identification and structural characterization.The anti-EC activity of dioscin was initially determined based on the inhibition of Ishikawa cell proliferation and tumor growth.The high-throughput sequencing data indicated that dioscin not only promoted apoptosis,including decrease of poly ADP-ribose polymerase(PARP)and B-cell lymphoma-2(Bcl-2)and increase of c-PARP and Bcl-2-associcated agonist of cell death(Bad),but also induced autophagy,including increase of autophagic lysosomes and LC3II/LC3I ratio.Mechanistic exploration suggested that dioscin induced autophagy and apoptosis through inhibition of PI3K/AKT/mTOR signaling pathway.Besides,the dioscin-regulated p53 pathway was mainly involved in autophagy induction.Furthermore,inhibition of Ishikawa cell autophagy was linked to dioscin-induced apoptosis.Our data suggest the immense potential of dioscin for the development of functional food for EC and related medical application.

DioscinIshikawa cellApoptosisAutophagyPI3K/AKTp53

Xiaoli Li、Runhui Ma、Zhijing Ni、Wei Wang、Kiran Thakur、Jianguo Zhang、Zhaojun Wei

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School of Biological Science and Engineering,North Minzu University,Yinchuan 750021,China

School of Food and Biological Engineering,Hefei University of Technology,Hefei 230009,China

School of Public Health,Anhui University of Science and Technology,Huainan 232001,China

National Key Research&Development Program of ChinaNational Natural Science Foundation of Ningxia ProvinceNational Natural Science Foundation of Ningxia ProvinceKey research and development projects in Ningxia province

2022YFF11003052021AAC020192022AAC032302021BEF02013

2024

10.26599/FSHW.2022.9250209

食品科学与人类健康(英文)

食品科学与人类健康(英文)

CSTPCD
ISSN：
年,卷(期)：2024.13(5)