Anti-biofilm and anti-virulence potential of cell free supernatant of Akkermansia muciniphila against Salmonella

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外文摘要：Akkermansia muciniphila is one of the commensals residing within the mammalian gut and co-evolving with the host.Numerous studies have demonstrated the benefits of A.muciniphila in ameliorating metabolic disorders,while little is known about the antimicrobial potential of A.muciniphila against pathogens.Here,we examined the antimicrobial and anti-virulence properties of cell free supernatant(CFS)of A.muciniphila against Salmonella Typhimurium.CFS retarded bacterial growth and inhibited the motility of S.Typhimurium SL1344 and S.Typhimurium 14028.CFS dose-dependently reduced cell hydrophobicity and auto-aggregation of both strains.Also,CFS from A.muciniphila significantly attenuated biofilm formation.Compared with untreated bacteria,CFS-treated bacteria significantly decreased adhesion and invasion to Caco-2 cells,and reduced intracellular survival in macrophages.CFS maintained antimicrobial properties after treatment with high temperatures and various proteases,while it lost its antimicrobial activity after pH neutralization.Gas chromatography-mass spectrometry(GC-MS)confirmed that A.muciniphila produced a certain amount of acetate and propionate,and ultra-high-performance liquid chromatography-mass spectrometry(UHPLC-MS)identified other organic acids and metabolites in CFS.In summary,CFS from A.muciniphila exhibited anti-biofilm and anti-virulence properties against Salmonella and could be potentially utilized in the food industry for controlling Salmonella contamination and reducing infection.

外文关键词：

Akkermansia muciniphilaSalmonellaBiofilmMotilityShort-chain fatty acidAdhesionInvasion

作者：

Jiaxiu Liu、Wenxiu Zhu、Huanhuan Liu、Xiaomeng Ren、Ningbo Qin、Xiaodong Xia

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作者单位：

National Engineering Research Center of Seafood,School of Food Science and Technology,Dalian Polytechnic University,Dalian 116034,China

基金：

National Key Research and Development Program of ChinaScience and Technology Research Program of the Liaoning Department of EducationShaanxi Key Research and Development projectLiaoNing Revitalization Talents Program

项目编号：

2022YFD2100104J20200442019SF-259XLYC1807220

出版年：

2024

DOI：

10.26599/FSHW.2022.9250215

食品科学与人类健康(英文)

CSTPCD

ISSN：

年,卷(期)：2024.13(5)