首页|Adjuvant postbiotic administration improves dental caries prognosis by restoring the oral microbiota

Adjuvant postbiotic administration improves dental caries prognosis by restoring the oral microbiota

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Conventional filling therapy fails to fundamentally reduce oral cariogenic bacteria.Thus,oral microbiota follow-up intervention after filling would be necessary for improving dental caries prognosis.We recruited 9 caries-free individuals,and 89 dental caries subjects(5 dropouts).Eighty-nine patients were randomized into three groups:caries(n=8;no treatment),control(n=40;filling),and postbiotics(n=41;filling and 14-day Probio-Eco® intervention).Salivary samples were collected at 0 day(after filling)and 14 days.Our results showed that the diversity of dental caries oral microbiota was significantly increased compared with healthy subjects,and filling could restore a healthier oral microbiota partially and temporarily.Thepostbiotics intervention keeps a low alpha-diversity.Co-occurrence network analysis showed that a more stable oral microbiota structure after postbiotics intervention.Taxonomic and functional annotation of the microbiota revealed that postbiotics co-treatment significantly:increased the relative abundance of Pseudomonas and P.reactans,decreased the relative abundance of Prevotella shahii,and enriched the energy metabolism-related pathways.BugBase-predicted phenotypes inferred to an oral microbiota with decreased potential pathogenic bacteria and increased oxidative stress-tolerant bacteria after postbiotics intervention.Collectively,it suggested that postbiotics co-treatment could be a promising strategy that restores the oral microecological balance for dental caries.

PostbioticsOral microbiotaSalivary microbiotaDental cariesPacific Biosciences(PacBio)single molecule real-time(SMRT)sequencingDental filling

Qing Liu、Teng Ma、Cuijiao Feng、Yalin Li、Hao Jin、Xuan Shi、Lai-Yu Kwok、Yan Shi、Tingtao Chen、Heping Zhang

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Affiliated Stomatological Hospital of Nanchang University,Nanchang 330000,China

Department of Endodontics,Affiliated Stomatological Hospital of Nanchang University

The Key Laboratory of Oral Biomedicine of Jiangxi Province

Jiangxi Province Clinical Research Center for Oral Diseases,Nanchang 330000,China

Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering,Inner Mongolia Agricultural University,Hohhot 010000,China

Key Laboratory of Dairy Products Processing,Ministry of Agriculture and Rural Affairs,Inner Mongolia Agricultural University,Hohhot 010000,China

Key Laboratory of Dairy Biotechnology and Engineering,Ministry of Education,Inner Mongolia Agricultural University,Hohhot 010000,China

National Engineering Research Center for Bioengineering Drugs and the Technologies,Institute of Translational Medicine,Nanchang University,Nanchang 330000,China

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National Natural Science Foundation of ChinaChina Agriculture Research System of MOF and MARAScience and Technology Major Projects of Inner Mongolia Autonomous Region

317201039112021ZD0014

2024

10.26599/FSHW.2022.9250217

食品科学与人类健康(英文)

食品科学与人类健康(英文)

CSTPCD
ISSN：
年,卷(期)：2024.13(5)