High β-sitosterol-D-glucoside content in sweet potato varieties and its anti-breast cancer potential through multiple metastasis-associated signals

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外文摘要：β-Sitosterol-D-glucoside(β-SDG)is a phytosterol compound whose antitumor activity has been confirmed by previous studies.However,its suppression on breast cancer remains unclear.To that purpose,we isolated β-SDG from sweet potato and investigated the breast-cancer-inhibiting mechanism using proteomic analysis.The sweet potato species S6 with high β-SDG content were chosen form 36 species and β-SDG was isolated by HPLC.Afterwards,an in situ animal model of breast cancer was established,and β-SDG significantly reduced the tumor volume of MCF-7 xenograft mice.Proteomic analysis of tumor tissues revealed that 127 of these proteins were upregulated and 80 were downregulated.Gene ontology and network analysis showed that regulatory proteins were mainly associated with epithelial-mesenchymal transition(EMT),myogenesis,cholesterol homeostasis,oxidative phosphorylation and reactive oxygen pathways,while Vimentin,NDUF,VDAC1,PPP2CA and SNx9 were the most significant 5 node degree genes.Meanwhile,in vitro and in vivo results showed that the protein expression of PPP2CA and Vimentin,which are markers of EMT,were involved in breast cancer cell metastasis and could be reversed by β-SDG.This work highlights β-SDG as a bioactive compound in sweet potato and the potential therapeutic effect of β-SDG for the treatment of breast cancer by inhibiting metastasis.

外文关键词：

β-Sitosterol-D-glucoside(β-SDG)Sweet potatoBreast cancerProteomicMetastasis

作者：

Jinyin Zhang、Lu Huang、Xiang Tang、Miao Pan、Xiaoli Ye、Xuegang Li、Hang Ma

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作者单位：

School of Pharmaceutical Sciences,Southwest University,Chongqing 400716,China

School of Life Sciences,Southwest University,Chongqing 400715,China

基金：

Special Key project of Technology Innovation and Application Development in ChongqingSpecial Key project of Technology Innovation and Application Development in ChongqingScience Foundation of School of Life Sciences SWUCounty-University Cooperation Innovation Funds of Southwest University

项目编号：

CSTC2021jscxgksb-N0033CSTB2021TIAD-KPX008520212005425201SZ202102

出版年：

2024

DOI：

10.26599/FSHW.2022.9250225

食品科学与人类健康(英文)

CSTPCD

ISSN：

年,卷(期)：2024.13(5)